2. Academic Validation
  3. ScRNA-seq reveals trained immunity-engaged Th17 cell activation against Edwardsiella piscicida-induced intestinal inflammation in teleost

ScRNA-seq reveals trained immunity-engaged Th17 cell activation against Edwardsiella piscicida-induced intestinal inflammation in teleost

  • Microbiol Res. 2024 Dec:289:127912. doi: 10.1016/j.micres.2024.127912.
Jin Yang 1,  Shu Cui 1,  Boning Shao 1,  Yanbo Zhao 1,  Zhuang Wang 1,  Qin Liu 2,  Yuanxing Zhang 3,  Dahai Yang 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Bioreactor Engineering, Laboratory for Aquatic Animal Diseases, East China University of Science and Technology, Shanghai 200237, China.
  • 2 State Key Laboratory of Bioreactor Engineering, Laboratory for Aquatic Animal Diseases, East China University of Science and Technology, Shanghai 200237, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China.
  • 3 State Key Laboratory of Bioreactor Engineering, Laboratory for Aquatic Animal Diseases, East China University of Science and Technology, Shanghai 200237, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China.
  • 4 State Key Laboratory of Bioreactor Engineering, Laboratory for Aquatic Animal Diseases, East China University of Science and Technology, Shanghai 200237, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China. Electronic address: [email protected].
PMID: 39326350 DOI: 10.1016/j.micres.2024.127912
Abstract

Mucosal immunity typically involves innate and adaptive immune cells, while the cellular mechanism of teleost's intestinal immune cells that engages gut homeostasis against Bacterial infection remains largely unknown. Taking advantage of the enteric fish pathogen (Edwardsiella piscicida) infection-induced intestinal inflammation in turbot (Scophthalmus maximus), we find that β-glucan training could mitigate the Bacterial infection-induced intestinal inflammation. Through single-cell transcriptome profiling and cellular function analysis, we identify that E. piscicida Infection could tune down the activation of intestinal Th17 cells, while β-glucan-training could preserve the potential to amplify and restore the function of intestinal Th17 cells. Moreover, through pharmacological inhibitor treatment, we identify that Th17 cells are essential for ameliorating Bacterial infection-induced intestinal inflammation in teleost. Taken together, these results suggest a new concept of trained immunity activation to regulate the intestinal Th17 cells' function, which might contribute to better developing strategies for maintaining gut homeostasis against Bacterial infection.

Keywords

Edwardsiella piscicida; Intestinal inflammation; Teleost; Th17 cells; β-Glucan.

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