2. Academic Validation
  3. Examining transfer of TERT to mitochondria under oxidative stress

Examining transfer of TERT to mitochondria under oxidative stress

  • Sci Rep. 2024 Oct 15;14(1):24185. doi: 10.1038/s41598-024-75127-4.
Dmitrii Burkatovskii 1,  Andrey Bogorodskiy 1,  Ivan Maslov 1,  Olga Moiseeva 1 2,  Roman Chuprov-Netochin 1,  Ekaterina Smirnova 1,  Nikolay Ilyinsky 1,  Alexey Mishin 1,  Sergey Leonov 1 3,  Georg Bueldt 1,  Valentin Gordeliy 1,  Thomas Gensch 4,  Valentin Borshchevskiy 5
Affiliations

Affiliations

  • 1 Moscow Institute of Physics and Technology (MIPT), 9 Institutsky lane, Dolgoprudny, 141700, Russian Federation.
  • 2 G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", 5 Prospekt Nauki, Pushchino, 142290, Russian Federation.
  • 3 Institute of Cell Biophysics, Russian Academy of Sciences, Institutskaya st., Pushchino, 142290, Russian Federation.
  • 4 Laboratory for Photochemistry and Spectroscopy, Division for Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, 3001, Leuven, Belgium.
  • 5 Moscow Institute of Physics and Technology (MIPT), 9 Institutsky lane, Dolgoprudny, 141700, Russian Federation. [email protected].
PMID: 39406807 DOI: 10.1038/s41598-024-75127-4
Abstract

The primary role of Telomerase is the lengthening of telomeres. Nonetheless, emerging evidence highlights additional functions of Telomerase outside of the nucleus. Specifically, its catalytic subunit, TERT (Telomerase Reverse Transcriptase), is detected in the cytosol and mitochondria. Several studies have suggested an elevation in TERT concentration within mitochondria in response to oxidative stress. However, the origin of this mitochondrial TERT, whether transported from the nucleus or synthesized de novo, remains uncertain. In this study, we investigate the redistribution of TERT, labeled with a SNAP-tag, in response to oxidative stress using laser scanning fluorescence microscopy. Our findings reveal that, under our experimental conditions, there is no discernible transport of TERT from the nucleus to the mitochondria due to oxidative stress.

Keywords

Mitochondria; Oxidative stress; Protein transport; SNAP-tag; TERT.

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