2. Academic Validation
  3. Complex regulation of Citron OGD2-dependent resistance to citrus canker caused by Xanthomonas citri subsp. citri

Complex regulation of Citron OGD2-dependent resistance to citrus canker caused by Xanthomonas citri subsp. citri

  • Plant Cell. 2025 Oct 8;37(10):koaf225. doi: 10.1093/plcell/koaf225.
Chenxing Hao 1 2 3,  Yan Jin 1 2 3,  Hanying Su 1 2 3,  Jianming Luo 1 2 3,  Xuzhao Luo 1 2 3,  Mingzhu Yao 1 2 3,  Yuting Song 1 2 3,  Jian Han 2 4,  Xiubin Liu 1 2,  Yu Xu 1 3,  Yajie Zheng 1 2,  Zhengmin Yang 1 2 3,  Dazhi Li 1 2 3,  Xuncheng Liu 5,  Shunyuan Xiao 6,  Xingyao Xiong 2,  Ziniu Deng 1 3 7,  Yunlin Cao 1 2 3,  Xianfeng Ma 1 2 3
Affiliations

Affiliations

  • 1 Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Hunan Agricultural University, Changsha 410128, China.
  • 2 Yuelushan Laboratory, Changsha 410128, China.
  • 3 National Center for Citrus Improvement, College of Horticulture, Hunan Agricultural University, Changsha 410128, China.
  • 4 Hunan Horticultural Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
  • 5 Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
  • 6 Institute for Bioscience and Biotechnology Research & Department of Plant Sciences and Landscape Architecture, University of Maryland College Park, Rockville, MD 20850, USA.
  • 7 NanLing Institute of Citrus Industry, Chenzhou 423000, China.
PMID: 41066433 DOI: 10.1093/plcell/koaf225
Abstract

Iron is an essential nutrient for all organisms. Feruloyl-COA 6-hydroxylase 1 (F6'H1) plays a pivotal role in iron uptake in plant roots by catalyzing the biosynthesis of iron-mobilizing scopoletin, a secondary metabolite also possessing antimicrobial activity. However, it remains unclear whether F6'H1-mediated iron uptake affects plant resistance to foliar pathogens and how such a process might be regulated. Here, we show that enhanced expression of 2-oxoglutarate-dependent dioxygenases 2 (CmOGD2), a homolog of F6'H1 in Citron C-05 (Citrus medica L.), confers resistance to citrus canker caused by Xanthomonas citri subsp. citri (Xcc). CmOGD2-mediated pathogen resistance is achieved by promoting iron uptake and the accumulation of Reactive Oxygen Species (ROS), which likely results in Ferroptosis. Furthermore, CmOGD2 interacts with the Enolase CmENO2 to destabilize CmZAT10.1, a transcriptional activator of CmOGD2, thereby forming a negative feedback loop that limits CmOGD2 expression. Notably, the Xcc effector pthA4 interferes with the CmOGD2-CmENO2 interaction, likely via a decoy mechanism, leading to CmZAT10.1 accumulation. These findings reveal complex regulatory mechanisms underlying the critical role of CmOGD2 in mediating Xcc resistance through iron- and ROS-dependent Ferroptosis.

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