2. Academic Validation
  3. The artificial chemical Y21 serves as a GA-signaling agonist and an auxin analog to promote seed germination and root development

The artificial chemical Y21 serves as a GA-signaling agonist and an auxin analog to promote seed germination and root development

  • J Integr Plant Biol. 2025 Nov 16. doi: 10.1111/jipb.70068.
Jiahui Xu 1 2,  Xiaofeng Luo 1,  Shaowei Wei 1,  Yueni Fan 1,  Zhikun Yang 2 3,  Xiaoting Zhao 1,  Mingyu Xia 1,  Baoshan Xian 1,  Xiaojing Xu 1,  Yali Liu 1,  Yiping Shi 1,  Mengyuan Ren 1,  Juan He 1,  Lei Wang 1,  Wenguan Zhou 1,  Weiming Tan 2,  Kai Shu 1 4
Affiliations

Affiliations

  • 1 Shaanxi Key Laboratory of Qinling Ecological Intelligent Monitoring and Protection, School of Ecology and Environment, Northwestern Polytechnical University, Xi'an, 710129, China.
  • 2 Engineering Research Center of Plant Growth Regulator, Ministry of Education & College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
  • 3 College of Pharmaceutical Science & Green Pharmaceutical Collaborative Innovation Center of Yangtze River Delta Region, Zhejiang University of Technology, Hangzhou, 310014, China.
  • 4 Research & Development, Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen, 518057, China.
PMID: 41243234 DOI: 10.1111/jipb.70068
Abstract

Gibberellins (GAs) and Auxin play central regulatory roles in seed germination and root system development, respectively, so that the application of these phytohormones to crops would be worthwhile, with an increasing potential demand in agriculture. However, there are few effective chemicals that simultaneously enhance both GA and Auxin signaling. Here, we report on an artificial thiourea derivative chemical, Y21, that serves as both a GA-signaling agonist and an Auxin analog, promoting seed germination and root development, as well as low-phosphorus tolerance. Phenotypic, biochemical, and genetic evidence demonstrated that Y21 enhances the interaction between GA and its receptor GID1C via the Val239 amino acid residue and consequently promotes degradation of the DELLA proteins REPRESSOR OF ga1-3 (RGA) and RGA-LIKE 2. Furthermore, we found that Y21 interacts with the Auxin receptor TIR1 via the Cys405 residue and thus promotes the turnover of the auxin-responsive Aux/IAA proteins. Consequently, Y21 significantly increases low-phosphorus tolerance of treated Plants by positively regulating lateral root development. To our knowledge, Y21 is the first GA-signaling agonist to be identified, and our results also demonstrate that this potent synthetic chemical, identified by chemical genetic screening, is effective at modulating plant development and stress tolerance.

Keywords

artificial chemical; auxin; gibberellins; seed germination; stress.

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