2. Academic Validation
  3. Arachidin-2 suppresses Peronophythora litchii and postharvest decay of litchi fruit

Arachidin-2 suppresses Peronophythora litchii and postharvest decay of litchi fruit

  • Food Chem. 2026 Jan 1;498(Pt 2):147298. doi: 10.1016/j.foodchem.2025.147298.
Bailin Li 1,  Xueying Meng 1,  Fen Liu 1,  Bao Yang 1,  Sui Kiat Chang 2,  Yueming Jiang 3
Affiliations

Affiliations

  • 1 South China National Botanical Garden, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • 2 Department of Allied Health Sciences, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia; Centre for Biomedical and Nutrition Research, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia. Electronic address: [email protected].
  • 3 South China National Botanical Garden, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: [email protected].
PMID: 41308211 DOI: 10.1016/j.foodchem.2025.147298
Abstract

Litchi downy blight, caused by Peronophythora litchii (P. litchii), is a devastating disease to litchi fruit. The objective of this study was to investigate the effects against P. litchii of arachidin-2 (ACD2), a naturally occurring secondary metabolite from peanuts. ACD2 inhibited the mycelial growth and sporangia germination with scanning electron microscopy showing deformed mycelia and sporangia in-vitro. ACD2 also suppressed the expression of pathogenic genes. Metabolomic analysis exhibited that ACD2 treatment significantly reduced levels of 17 metabolites, including nicotinamide adenine dinucleotide (NAD) and adenosine triphosphate (ATP), while increasing cyclic adenosine monophosphate (cAMP) content. Molecular docking suggested that ACD2 binds strongly to alternative oxidase (AOX), inhibiting the enzyme. Moreover, pyruvate, an AOX activator, counteracted the ACD2's effects on ATP content, hydrogen peroxide level, mycelial growth and leaf lesion. ACD2 reduced disease severity and inhibited browning via the pathogen-induced litchi fruit in-vivo. These findings suggested that ACD2 is a promising biocontrol agent against litchi downy blight.

Keywords

Arachidin-2; Litchi downy blight; Litchi fruit; Metabolomic analysis; Peronophythora litchi.

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