2. Academic Validation
  3. Z11, a small-molecule inhibitor of NAP1L1, alleviates cardiac fibrosis by regulating AKT/mTOR pathway

Z11, a small-molecule inhibitor of NAP1L1, alleviates cardiac fibrosis by regulating AKT/mTOR pathway

  • Eur J Pharmacol. 2026 Jan 12:1011:178440. doi: 10.1016/j.ejphar.2025.178440.
Tong Yu 1,  Haoshu Wang 1,  Jingyi Gong 1,  Jun Ma 1,  Chengyu Kong 1,  Yaoxuan He 1,  Xinjie Zhou 1,  Shuxia Jiang 1,  Ping Pang 1,  Zhicheng Zuo 2,  Yuhong Zhou 3,  Hongli Shan 4
Affiliations

Affiliations

  • 1 Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Noncoding RNA, Institute for Frontier Medical Technology, College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, 201620, PR China.
  • 2 Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Noncoding RNA, Institute for Frontier Medical Technology, College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, 201620, PR China. Electronic address: [email protected].
  • 3 State Key Laboratory of Frigid Zone Cardiovascular Diseases (SKLFZCD), Harbin Medical University, Harbin, Heilongjiang, 150081, PR China; Department of Basic Medicine, Institute of Respiratory Diseases Xiamen Medical College, Xiamen Medical College, Xiamen, Fujian, 361023, PR China. Electronic address: [email protected].
  • 4 Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Noncoding RNA, Institute for Frontier Medical Technology, College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, 201620, PR China; State Key Laboratory of Frigid Zone Cardiovascular Diseases (SKLFZCD), Harbin Medical University, Harbin, Heilongjiang, 150081, PR China. Electronic address: [email protected].
PMID: 41354299 DOI: 10.1016/j.ejphar.2025.178440
Abstract

Cardiac fibrosis represents the terminal pathological progression of diverse cardiovascular diseases, characterized by aberrant activation and migration of cardiac fibroblasts, as well as excessive and disordered deposition of the extracellular matrix. Our previous study showed that NAP1L1 is an important regulator of cardiac fibrosis and is upregulated in ischemic cardiomyopathy patient hearts. Accordingly, discovery of NAP1L1 inhibitors and elucidating their underlying mechanisms of anti-cardiac fibrosis should be urgently needed. Herein, we identified a new NAP1L1 small molecule inhibitor Z1149421873 (named Z11) by the structure-based drug design strategy. Z11 was shown to inhibit cardiac fibroblasts activation, deposition of Collagen hypersecretion, and alleviate cardiac fibrosis in both in vitro models induced by TGF-β1 and in vivo myocardial infarction (MI) mouse models. Mechanistically, Z11 interfered with the interaction between NAP1L1 and YAP1, which in turn promotes the ubiquitination degradation of YAP1, thereby inhibiting the Akt/mTOR signaling pathway, and attenuating myofibroblast activity and cardiac fibrosis, and improving cardiac function after MI. This finding may provide new insights for the development of promising candidate drugs for the treatment of cardiac fibrosis related diseases in the future.

Keywords

AKT/mTOR pathway; Cardiac fibrosis; NAP1L1; YAP1 ubiquitination; Z1149421873.

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