2. Academic Validation
  3. LED blue light photobiomodulation induces ferroptosis and apoptosis via ROS-mediated oxidative damage in osteosarcoma cells

LED blue light photobiomodulation induces ferroptosis and apoptosis via ROS-mediated oxidative damage in osteosarcoma cells

  • J Photochem Photobiol B. 2026 Jan:274:113350. doi: 10.1016/j.jphotobiol.2025.113350.
Jiali Yang 1,  Haokuan Qin 2,  Xiaojing Miao 3,  Longfei Huo 3,  Qiqi Fu 3,  Hui Jiang 2,  Jianfeng Niu 4,  Muqing Liu 5
Affiliations

Affiliations

  • 1 Jiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang 222005, China; School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China; School of Information Science and Technology, Fudan University, Shanghai 200438, China.
  • 2 Academy for Engineering and Technology, Fudan University, Shanghai 200433, China.
  • 3 School of Information Science and Technology, Fudan University, Shanghai 200438, China.
  • 4 School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China.
  • 5 School of Information Science and Technology, Fudan University, Shanghai 200438, China; Zhongshan-Fudan Joint Innovation Center, Zhongshan 528400, China. Electronic address: [email protected].
PMID: 41455154 DOI: 10.1016/j.jphotobiol.2025.113350
Abstract

Blue light (400-500 nm) photobiomodulation (PBM), particularly utilizing light-emitting diodes (LEDs), has garnered extensive attention for its therapeutic efficacy across various conditions, including oncology. Nevertheless, its impact on osteosarcoma cells remain inadequately characterized. This study sought to investigate the tumor-suppressive properties and underlying molecular mechanisms of LED blue light PBM in human osteosarcoma cell lines HOS and MG63. The results indicated that blue light PBM effectively suppressed cell proliferation and triggered G2/M phase cell cycle arrest. Moreover, blue light PBM activated Apoptosis and Ferroptosis, characterized by significant changes in ferroptosis-associated proteins (HO-1, PTGS2, GPX4). Concurrently, ROS accumulation triggered oxidative stress, as demonstrated by increased MDA and LPO levels, well-established markers of Ferroptosis. These findings underscore the promising potential of LED blue light PBM as an innovative, non-pharmacological therapeutic strategy for osteosarcoma, warranting further investigation for future clinical applications.

Keywords

Apoptosis; Blue light PBM; Ferroptosis; Osteosarcoma cells; ROS.

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