2. Academic Validation
  3. The LincRNA8058-mediated chi-miR-342-5p/SCARA5 axis regulates milk fat metabolism in dairy goats

The LincRNA8058-mediated chi-miR-342-5p/SCARA5 axis regulates milk fat metabolism in dairy goats

  • Int J Biol Macromol. 2026 Jan;340(Pt 2):150254. doi: 10.1016/j.ijbiomac.2026.150254.
Mengke Ni 1,  Wanting Wang 1,  Tiantian Xiong 1,  Chong Chen 1,  Meiwen Song 1,  Ziting Yue 1,  Jun Luo 1,  Huaiping Shi 1,  Cong Li 2
Affiliations

Affiliations

  • 1 College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
  • 2 College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China. Electronic address: [email protected].
PMID: 41539521 DOI: 10.1016/j.ijbiomac.2026.150254
Abstract

To investigate the regulatory role of lncRNAs in milk fat metabolism in dairy goats, mammary gland tissues from six Saanen dairy goats, with consistently high (above 4.19%) and low (below 2.90%) milk fat percentages, were used as the subjects of this study. Through whole-transcriptome Sequencing of mammary tissue from lactating dairy goats, we observed that linc8058 is highly expressed throughout lactation and positively regulates milk fat anabolism. Functional assays confirmed that linc8058 competitively binds with chi-miR-342-5p, modulating the expression of the target scavenger receptor class A member 5 (SCARA5). Interference with linc8058 or the use of chi-miR-342-5p mimics led to reduced SCARA5 mRNA and protein levels and inhibited the activity of the PI3K/Akt signaling pathway, resulting in decreased milk fat secretion by mammary epithelial cells in dairy goats. These findings indicate that linc8058 is a positive regulator of milk fat anabolism in dairy goats.

Keywords

Chi-miR-342-5p; Dairy goats; Linc8058; Milk fat metabolism; PI3K/AKT.

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