2. Academic Validation
  3. Development of Organogel-AFPT System for Encapsulation and Cryopreservation of Yarrowia lipolytica

Development of Organogel-AFPT System for Encapsulation and Cryopreservation of Yarrowia lipolytica

  • ACS Appl Mater Interfaces. 2026 Feb 11;18(5):8807-8815. doi: 10.1021/acsami.5c22113.
Ziying Zhang 1,  Ruiqi Cui 2,  Haoyu Yang 1,  Xiangyu Zhang 1,  Lei Zhang 1 3
Affiliations

Affiliations

  • 1 Department of Bioengineering, School of Synthetic Biology and Biomanufacturing, State Key Laboratory of Synthetic Biology, and Frontier Science Center for Synthetic Biology, Tianjin University, Tianjin 300350, China.
  • 2 Corpus Christi College, University of Cambridge, Cambridge CB2 1RH, United Kingdom.
  • 3 Haihe Laboratory of Sustainable Chemical Transformations, Tianjin 300192, China.
PMID: 41591409 DOI: 10.1021/acsami.5c22113
Abstract

Encapsulation of Microorganisms is a promising strategy in biomedical and industrial applications to enhance stability, reusability, and functionality. However, the cryopreservation of microorganism-encapsulated Materials still represents a formidable challenge because of the mechanical and osmotic damage caused by inevitable ice formation at subzero temperatures. In this study, we developed a hydrophobic antifreeze material (organogel-AFPT) specifically for oleaginous Microorganisms, following the principle of reducing the overall water content of the system and inhibiting ice nucleation and growth. By the cryoprotection of organogel-AFPT, 94% survival rate of Yarrowia lipolytica (Y. lipolytica) can be achieved after cryopreservation at -196 °C. It is also found that the organogel-AFPT can serve as a convenient platform for information encryption and decryption. The findings in this work provide a new strategy for the development of antifreeze living Materials.

Keywords

Yarrowia lipolytica; antifreeze peptides; cryopreservation; encapsulation; organogel.

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