Effect of Hydroxytyrosol on OTULIN Levels in Testıcular Tıssue in Experımental Dıabetes Model Induced wıth Streptozotocin

Diabetes mellitus (DM), a Metabolic Disease, is an important health problem with many complications, including male reproductive disorders. Hydroxytyrosol (HT), an active polyphenolic, is one of the compounds of olives, which has many proven health benefits. The purpose of this study was to ascertain the possible therapeutic benefits of HT on testicular tissue in model of DM caused by streptozotocin (STZ). In addition, the aim was to investigate the effects of HT on proinflammatory cytokines and OTULIN levels in testicular tissue in an experimental DM model. A total of 32 male rats were used in the 6-week study and the rats were divided equally into 4 groups. The control group was untreated, while rats in the DM group received a single dose of STZ to induce diabetes. After inducing experimental diabetes in the DM + HT group, 5 mg/kg/day Hydroxytyrosol was administered. The HT group received 5 mg/kg/day Hydroxytyrosol. After all treatments were completed, the experiment was terminated and testicular tissues were taken for analyses. Experimental DM model caused oxidative stress, histopathological changes, increase in pro-inflammatory cytokines, decrease in OTULIN levels and increase in apoptotic germ cells in testicular tissues. In contrast, HT supplementation to rats with experimental DM was found to modulate these adverse effects to a great extent. In conclusion, HT may have anti-inflammatory, anti-oxidant and anti-diabetic effects against DM and related damage in testicular tissues by regulating OTULIN levels.

Diabetes mellitus; Hydroxytyrosol; OTULIN; Testis.