Isolation of a human kappa opioid receptor cDNA from placenta

By using a mouse delta Opioid Receptor cDNA as a probe, a kappa Opioid Receptor gene was isolated from a human genomic library. Reverse Transcriptase/PCR was subsequently used to isolate the corresponding cDNA from human placenta mRNA. Characterization of the cloned receptor with a kappa agonist in transfected COS-7 cells, revealed a Kd of 0.67 nM, which is similar to rodent kappa 1 receptors. In competition binding experiments, non-kappa agonists had kis > 1000nM. Furthermore, when expressed in COS-7 cells, the kappa receptor is negatively linked to cAMP. A Northern blot showed the presence of two transcripts, 6 and 7 kB in size, in both placenta and brain mRNA, which hybridized to the kappa cDNA.