2. Academic Validation
  3. Hydrolysis of bound GTP by ARF protein triggers uncoating of Golgi-derived COP-coated vesicles

Hydrolysis of bound GTP by ARF protein triggers uncoating of Golgi-derived COP-coated vesicles

  • J Cell Biol. 1993 Dec;123(6 Pt 1):1365-71. doi: 10.1083/jcb.123.6.1365.
G Tanigawa 1,  L Orci,  M Amherdt,  M Ravazzola,  J B Helms,  J E Rothman
Affiliations

Affiliation

  • 1 Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021.
PMID: 8253837 DOI: 10.1083/jcb.123.6.1365
Abstract

The cycle of nucleotide exchange and hydrolysis by a small GTP-binding protein, ADP-ribosylation factor (ARF), helps to provide vectoriality to vesicle transport. Coat assembly is triggered when ARF binds GTP, initiating transport vesicle budding, and coat disassembly is triggered when ARF hydrolyzes GTP, allowing the uncoated vesicle to fuse.

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