2. Academic Validation
  3. Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor

Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor

  • FEBS Lett. 1993 Feb 8;317(1-2):139-42. doi: 10.1016/0014-5793(93)81509-x.
N Vita 1,  P Laurent,  S Lefort,  P Chalon,  X Dumont,  M Kaghad,  D Gully,  G Le Fur,  P Ferrara,  D Caput
Affiliations

Affiliation

  • 1 Unité Biochimie des Protéines, Sanofi Elf Biorecherches, Labège, France.
PMID: 8381365 DOI: 10.1016/0014-5793(93)81509-x
Abstract

A human Neurotensin Receptor (hNTR) cDNA was cloned from the colonic adenocarcinoma cell line HT29. The cloned cDNA encodes a putative peptide of 418 Amino acids with 7 transmembrane domains. The amino acid sequence of the hNTR is 84% identical to the rat NTR [Neuron, 4 (1990) 847-854]. Transfection of this cDNA into COS cells results in the expression of receptors with pharmacological properties similar to those found with HT29 cells. Northern blot analysis using the hNTR cDNA probe indicated a single transcript of 4 kb in the brain, the small intestine and blood mononuclear cells.

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