2. Academic Validation
  3. cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase enzyme

cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase enzyme

  • Biosci Biotechnol Biochem. 1998 May;62(5):1037-40. doi: 10.1271/bbb.62.1037.
K Yanagisawa 1,  Y Sakakibara,  M Suiko,  Y Takami,  T Nakayama,  H Nakajima,  K Takayanagi,  Y Natori,  M C Liu
Affiliations

Affiliation

  • 1 Department of Biochemistry, University of Texas Health Center, Tyler 75710, USA.
PMID: 9648242 DOI: 10.1271/bbb.62.1037
Abstract

A cDNA encoding the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate (APS) kinase was cloned and sequenced. The Enzyme contains an APS kinase domain in its N-terminal portion and an ATP sulfurylase domain in its C-terminal portion. Recombinant full-length Enzyme and its constituent APS kinase and ATP sulfurylase domains were individually expressed, purified, and shown to have their respective enzymatic activities.

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