1. NF-κB
  2. Keap1-Nrf2
  3. ML334

ML334 (Synonyms: LH601A)

Cat. No.: HY-110258 Purity: >99.0%
Handling Instructions

ML334 is a potent, cell permeable activator of NRF2 by inhibition of Keap1-NRF2 protein-protein interaction. ML334 binds to Keap1 Kelch domain with a Kd of 1 μM. ML334 stimulates NRF2 expression and nuclear translocation and induces antioxidant response elements (ARE) activity.

For research use only. We do not sell to patients.

ML334 Chemical Structure

ML334 Chemical Structure

CAS No. : 1432500-66-7

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10 mM * 1 mL in DMSO USD 545 Get quote
1 mg USD 185 In-stock
Estimated Time of Arrival: December 31
5 mg USD 555 Get quote
10 mg USD 950 Get quote
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Based on 1 publication(s) in Google Scholar

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Description

ML334 is a potent, cell permeable activator of NRF2 by inhibition of Keap1-NRF2 protein-protein interaction. ML334 binds to Keap1 Kelch domain with a Kd of 1 μM. ML334 stimulates NRF2 expression and nuclear translocation and induces antioxidant response elements (ARE) activity[1][2].

IC50 & Target

Ki: 1 μM (Keap1)[2]

In Vitro

ML334 (LH601A; 50-100 µM; 6-16 hours; HEK293 cells) treatment increases NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. And also enhances HO-1 mRNA expression between 4- and 7-fold at 6 h, with lesser fold-changes at 16 h[1].
ML334 (LH601A; 50-100 µM; 16 hours; HEK293 cells) treatment induces HO-1 and TRX1 proteins in HEK293 cells[1].
ML334 (LH601A) stimulates NRF2 expression and nuclear translocation in HEK293 cells[1].

RT-PCR[1]

Cell Line: HEK293 cells
Concentration: 50 μM or 100 µM
Incubation Time: 6 hours, 16 hours
Result: Increased NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. Enhanced HO-1 mRNA expression between 4- and 7-fold at 6 h.

Western Blot Analysis[1]

Cell Line: HEK293 cells
Concentration: 50 μM or 100 µM
Incubation Time: 16 hours
Result: Enhanced HO-1 and TRX1 protein expression at 16 h.
Molecular Weight

446.50

Formula

C₂₆H₂₆N₂O₅

CAS No.

1432500-66-7

SMILES

O=C(C1=CC=CC=C1C2=O)N2C[[email protected]@H]3C4=CC=CC=C4CCN3C([[email protected]]5[[email protected]](CCCC5)C(O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
References

Purity: >99.0%

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Keywords:

ML334LH601AML 334ML-334Keap1-Nrf2AntioxidantresponseelementsnucleartranslocationNQO1TRX1Inhibitorinhibitorinhibit

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ML334
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