1. Immunology/Inflammation
  2. NOD-like Receptor (NLR)
  3. NLRP3-IN-2

NLRP3-IN-2 

Cat. No.: HY-W011082 Purity: >98.0%
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NLRP3-IN-2, an intermediate substrate in the synthesis of glyburide, inhibits the formation of the NLRP3 inflammasome in cardiomyocytes and limits the infarct size following myocardial ischemia/reperfusion in the mouse, without affecting glucose metabolism.

For research use only. We do not sell to patients.

NLRP3-IN-2 Chemical Structure

NLRP3-IN-2 Chemical Structure

CAS No. : 16673-34-0

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10 mM * 1 mL in DMSO USD 50 In-stock
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100 mg USD 60 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

NLRP3-IN-2, an intermediate substrate in the synthesis of glyburide, inhibits the formation of the NLRP3 inflammasome in cardiomyocytes and limits the infarct size following myocardial ischemia/reperfusion in the mouse, without affecting glucose metabolism[1].

In Vivo

NLRP3-IN-2 is well tolerated with no effects on the glucose levels in vivo[1].
NLRP3-IN-2 (100 mg/kg) treatment in a model of AMI due to ischemia+reperfusion significantly inhibits the activity of inflammasome (caspase-1) in the heart by 90% (P<0.01) and reduced infarct size, measured at pathology (by >40%, P<0.01) and with troponin I levels (by >70%, P<0.01) [1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Experimental acute myocardial infarction (AMI) model in mice[1].
Dosage: 100 mg/kg.
Administration: Intraperitoneal administration 30 minutes prior to surgery, then every 6 hours for 3 additional doses.
Result: Led to a significant >90% reduction in caspase-1 activity (reflective of the formation of an active inflammasome) in the heart tissue measured 24 hours after ischemia.
Led to a significant reduction in the infarct size measured with TTC (>40% reduction) or troponin I levels (>70% reduction) when compared with vehicle alone.
Molecular Weight

368.84

Formula

C₁₆H₁₇ClN₂O₄S

CAS No.

16673-34-0

SMILES

O=C(NCCC1=CC=C(S(=O)(N)=O)C=C1)C2=CC(Cl)=CC=C2OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (338.90 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7112 mL 13.5560 mL 27.1120 mL
5 mM 0.5422 mL 2.7112 mL 5.4224 mL
10 mM 0.2711 mL 1.3556 mL 2.7112 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.64 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.64 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: >98.0%

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Keywords:

NLRP3-IN-2NOD-like Receptor (NLR)ischemiareperfusioninflammasomeacutemyocardialinfarctionInhibitorinhibitorinhibit

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NLRP3-IN-2
Cat. No.:
HY-W011082
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