CEACAM1 Protein, Human (N-His)

CEACAM1 protein is involved in various biological processes. It is phosphorylated by the insulin receptor (INSR) in response to insulin stimulation, thereby promoting insulin clearance and regulating hepatic lipogenesis. CEACAM1 Protein, Human (N-His) is the recombinant human-derived CEACAM1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CEACAM1 Protein, Human (N-His) is 394 a.a., with molecular weight of ~45 kDa.

The SF3R gene is involved in various biological processes and functions. It regulates insulin action by promoting the clearance of insulin and regulating lipogenesis in the liver. Upon insulin stimulation, SF3R undergoes phosphorylation by the insulin receptor (INSR), leading to increased insulin endocytosis and degradation. This results in a reduction of fatty acid synthesis. SF3R also plays a role in down-regulating cell proliferation through its interaction with SHC1, which decreases the coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 and phosphatidylinositol 3-kinase pathways.

Measured by its ability to inhibit IL-2 secretion by HuT 78 human cutaneous T cell lymphoma cells in the presence of anti-CD3. The ED₅₀ for this effect is 1.534 ng/mL, corresponding to a specific activity is 6.51×10⁵ U/mg.

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  Measured by its ability to inhibit IL-2 secretion by HuT 78 human cutaneous T cell lymphoma cells in the presence of anti-CD3 .The ED₅₀ for this effect is 1.534 ng/mL, corresponding to a specific activity is 6.51×10⁵ U/mg.

Human

E. coli

N-6*His

P13688 (Q35-G428)

634  [NCBI]

QLTTESMPFNVAEGKEVLLLVHNLPQQLFGYSWYKGERVDGNRQIVGYAIGTQQATPGPANSGRETIYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPETQDTTYLWWINNQSLPVSPRLQLSNGNRTLTLLSVTRNDTGPYECEIQNPVSANRSDPVTLNVTYGPDTPTISPSDTYYRPGANLSLSCYAASNPPAQYSWLINGTFQQSTQELFIPNITVNNSGSYTCHANNSVTGCNRTTVKTIIVTELSPVVAKPQIKASKTTVTGDKDSVNLTCSTNDTGISIRWFFKNQSLPSSERMKLSQGNTTLSINPVKREDAGTYWCEVFNPISKNQSDPIMLNVNYNALPQENGLSPG

Approximately 45 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.