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CEACAM1 Protein, Human (N-His)

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Based on 1 Customer Validation

CEACAM1 protein is involved in various biological processes. It is phosphorylated by the insulin receptor (INSR) in response to insulin stimulation, thereby promoting insulin clearance and regulating hepatic lipogenesis. CEACAM1 Protein, Human (N-His) is the recombinant human-derived CEACAM1 protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CEACAM1 Protein, Human (N-His)
  • Experimental Validation Results for CEACAM1 Protein, Human (N-His)
  • CEACAM1 Protein, Human (N-His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CEACAM1 protein is involved in various biological processes. It is phosphorylated by the insulin receptor (INSR) in response to insulin stimulation, thereby promoting insulin clearance and regulating hepatic lipogenesis. CEACAM1 Protein, Human (N-His) is the recombinant human-derived CEACAM1 protein, expressed by E. coli , with N-6*His labeled tag.

Background

The SF3R gene is involved in various biological processes and functions. It regulates insulin action by promoting the clearance of insulin and regulating lipogenesis in the liver. Upon insulin stimulation, SF3R undergoes phosphorylation by the insulin receptor (INSR), leading to increased insulin endocytosis and degradation. This results in a reduction of fatty acid synthesis. SF3R also plays a role in down-regulating cell proliferation through its interaction with SHC1, which decreases the coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 and phosphatidylinositol 3-kinase pathways.

Verified Bioactivity

Measured by its ability to inhibit IL-2 secretion by HuT 78 human cutaneous T cell lymphoma cells in the presence of anti-CD3. The ED50 for this effect is 1.534 ng/mL, corresponding to a specific activity is 6.51×105 U/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit IL-2 secretion by HuT 78 human cutaneous T cell lymphoma cells in the presence of anti-CD3 .The ED50 for this effect is 1.534 ng/mL, corresponding to a specific activity is 6.51×105 U/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    P13688-1 (Q35-G428)

  • Gene ID
    634  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • CEACAM1 (Q35-G428)
      Accession # P13688-1
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CEACAM1; CD66a; Prev. BGP; Biliary Glycoprotein 1; BGP1; CEACAM1 Protein; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein); Antigen CD66; Carcinoembryonic Antigen Related Cell Adhesion Molecule 1; BGP-1; Cell Adhesion Molec

  • AA Sequence

    QLTTESMPFNVAEGKEVLLLVHNLPQQLFGYSWYKGERVDGNRQIVGYAIGTQQATPGPANSGRETIYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPETQDTTYLWWINNQSLPVSPRLQLSNGNRTLTLLSVTRNDTGPYECEIQNPVSANRSDPVTLNVTYGPDTPTISPSDTYYRPGANLSLSCYAASNPPAQYSWLINGTFQQSTQELFIPNITVNNSGSYTCHANNSVTGCNRTTVKTIIVTELSPVVAKPQIKASKTTVTGDKDSVNLTCSTNDTGISIRWFFKNQSLPSSERMKLSQGNTTLSINPVKREDAGTYWCEVFNPISKNQSDPIMLNVNYNALPQENGLSPG

  • Predicted Molecular Mass

    43.3 kDa

  • Molecular Weight

    Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, 200 mM arginine, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit IL-2 secretion by HuT 78 human cutaneous T cell lymphoma cells in the presence of anti-CD3 .The ED50 for this effect is 1.534 ng/mL, corresponding to a specific activity is 6.51×105 U/mg.

  • CEACAM1 Protein, Human (N-His) Product Image
    1/16
  • Experimental Validation Results for CEACAM1 Protein, Human (N-His)
  • Experimental Validation Results for CEACAM1 Protein, Human (N-His)
  • CEACAM1 Protein, Human (N-His) Product Image