1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. Epigen
  5. Epigen Protein, Human

Epigen Protein, Human

Cat. No.: HY-P7010
COA Handling Instructions

Epigen Protein, Human is a EGF-like growth factor that does not stimulate cells singly expressing ErbB-2, but acts as a mitogen for cells expressing ErbB-1 and co-expressing ErbB-2 in combination with the other ErbBs, with potent mitogenic activity.

For research use only. We do not sell to patients.

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10 μg $125 In-stock
50 μg $350 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

Epigen Protein, Human is a EGF-like growth factor that does not stimulate cells singly expressing ErbB-2, but acts as a mitogen for cells expressing ErbB-1 and co-expressing ErbB-2 in combination with the other ErbBs, with potent mitogenic activity.

Background

Recombinant Human Epigen is a EGF-like growth factor that does not stimulate cells singly expressing ErbB-2, but acts as a mitogen for cells expressing ErbB-1 and co-expressing ErbB-2 in combination with the other ErbBs, with potent mitogenic activity[1].

Biological Activity

The ED50 is <1 μg/mL as measured by 3T3 cells, corresponding to a specific activity of >1 × 103 units/mg.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

Q6UW88-2 (A24-A95)

Gene ID
Molecular Construction
N-term
Epigen (A24-A95)
Accession # Q6UW88-2
C-term
Synonyms
rHuEpigen; Epithelial mitogen; EPG
AA Sequence

AVTVTPPITAQQADNIEGPIALKFSHLCLEDHNSYCINGACAFHHELEKAICRCFTGYTGERCEHLTLTSYA

Molecular Weight

Approximately 8.1 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

Epigen Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Epigen Protein, Human
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HY-P7010
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