GMP IL-4 Protein, Human (HEK293)

GMP IL-4 Protein, Human (HEK293) is a recombinant GMP-grade protein produced by HEK293 cells with C-His tag. IL-4 is an anti-inflammatory cytokine acting as a pleiotropic regulator of numerous immune and inflammatory processes.

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a cytokine that plays an important role in regulating inflammation and immune responses. It is typically secreted by T helper 2 lymphocytes, mast cells, eosinophils, and basophils and plays a protective role in neurological disorders (e.g., multiple sclerosis, spinal cord injury). IL-4 induces the differentiation of naïve helper T cells to Th2 cells. This cytokine binds the IL-4 receptor that also binds another cytokine interleukin 13 (IL-13), which may explain the overlapping functions of IL-4 and IL-13. IL-4 application at injured nerves in mice shifted F4/80+ macrophages from the proinflammatory M1 to the antiinflammatory M2 phenotype, which synthesized opioid peptides (Met-enkephalin, β-endorphin, and dynorphin A 1-17)^[1][2].

The specific activity is >1×10⁷ U/mg.

Human

HEK293

Tag Free

P05112-1 (H25-S153)

3565  [NCBI]

HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

Approximately 18.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in injection water.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Le HV, et al. Isolation and characterization of multiple variants of recombinant human interleukin 4 expressed in mammalian cells. J Biol Chem. 1988 Aug 5;263(22):10817-23.  [Content Brief]

  [2]. Melih Ö Celik, et al. IL-4 induces M2 macrophages to produce sustained analgesia via opioids. JCI Insight. 2020 Feb 27;5(4):e133093.  [Content Brief]