hERG Protein, Human (HEK293, GFP, His)

The hERG protein is the α subunit of voltage-gated potassium channels and is critical for mediating cardiac delayed rectifier potassium current (IKr). It lacks individual channel activity but dynamically affects properties by forming heterotetramers with other isoforms. hERG Protein, Human (HEK293, GFP, His) is the recombinant human-derived hERG protein, expressed by HEK293 , with C-10*His, C-GFP labeled tag.

hERG, the pore-forming α subunit of the voltage-gated inwardly rectifying potassium channel, plays a pivotal role in mediating the rapidly activating component of the delayed rectifying potassium current in the heart (IKr). While it lacks channel activity on its own, hERG dynamically influences channel properties through the formation of heterotetramers with other isoforms. This subunit assembly not only modulates the channel characteristics but also leads to the intracellular retention of the heterotetramers, subsequently undergoing ubiquitin-dependent degradation. The intricate interplay of hERG with other isoforms highlights its regulatory function in the finely tuned orchestration of potassium currents crucial for cardiac physiology.

Human

HEK293

C-10*His;C-GFP

Q12809-1 (M1-S1159, ΔP141-A350 and ΔS871-R1005)

3757

Full Length

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.