1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-1R IL-31
  5. IL-31 Protein, Cynomolgus (137a.a, HEK293, His)

IL-31 Protein, Cynomolgus (137a.a, HEK293, His)

Cat. No.: HY-P77706
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

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IL-31 Protein, Cynomolgus (137a.a, HEK293, His) Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Cynomolgus

Source

HEK 293

Tag

His

Accession

G7PJ78 (L27-T163)

Gene ID

/

Synonyms
Interleukin-31; IL-31; IL31
AA Sequence

Molecular Weight

23-30 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE. Greater than 95% as determined by HPLC

Biological Activity
Human IL-31 RA captured on CM5 Chip via Protein A can bind Cynomolgus IL-31, His Tag with an affinity constant of 0.715 nM as determined in SPR assay (Biacore T200).
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-31 Protein, Cynomolgus (137a.a, HEK293, His)
Cat. No.:
HY-P77706
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