2. Metabolic Enzyme/Protease
  3. PI4P5K
  4. UNC3230

UNC3230 is a potent, selective and ATP-competitive PIP5K1C inhibitor with an IC50 of ~41 nM. UNC3230 also inhibits PIP4K2C and does not inhibit any of the other lipid kinases that regulate phosphoinositide levels. UNC3230 has antinociceptive and anticancer effects.

For research use only. We do not sell to patients.

CAS No. : 1031602-63-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 9 publication(s) in Google Scholar

UNC3230 Related Antibodies

Top Publications Citing Use of Products

    UNC3230 purchased from MedChemExpress. Usage Cited in: Exp Mol Med. 2024 Aug;56(8):1736-1749.  [Abstract]

    Western blot analysis of p62, LC3B-I, LC3B-II and GAPDH in the presence of UNC3230 (0.5 μM), apilimod and UNI418.

    UNC3230 purchased from MedChemExpress. Usage Cited in: Exp Mol Med. 2024 Aug;56(8):1736-1749.  [Abstract]

    Immunofluorescence staining with LC3B (green) and p62 (red) antibodies upon treatment with UNC3230 (0.5 μM).

    UNC3230 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Sep 30;24(19):14786.

    Colony formation assay was performed using HeLa cells treated with UNC3230 (100 nM) and/or the YAP inhibitor verteporfin (VP, 1 μM).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    UNC3230 is a potent, selective and ATP-competitive PIP5K1C inhibitor with an IC50 of ~41 nM. UNC3230 also inhibits PIP4K2C and does not inhibit any of the other lipid kinases that regulate phosphoinositide levels. UNC3230 has antinociceptive and anticancer effects[1].

    IC50 & Target

    IC50: ~41 nM (Phosphatidylinositol 4-phosphate 5 kinase type 1C (PIP5K1C))[1]
    In Vitro

    Membrane PIP2 levels are significantly reduced by ~45% in dorsal root ganglia (DRG) neurons treated with 100 nM UNC3230 (~2-fold above the IC50) relative to vehicle controls. UNC3230 significantly reduces lysophosphatidic acid (LPA)-evoked calcium signaling in cultured DRG neurons relative to vehicle[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    UNC3230 Related Antibodies
    In Vivo

    UNC3230 (2 nmol) significantly increases noxious heat-evoked paw withdrawal latency for two hours after intrathecal injection in wild-type mice, indicating an antinociceptive effect[1].
    UNC3230 (2 nmol; intrathecal injection) is administered then one hour later co-injected 1 nmol LPA with UNC3230 (2 nmol, intrathecal injection). UNC3230 significantly blunts thermal hyperalgesia and mechanical allodynia compared to vehicle[1].
    UNC3230 (2 nmol; intrathecal injection) significantly blunts thermal hyperalgesia and mechanical allodynia in the complete Freund’s adjuvant (CFA)-inflamed hindpaw (relative to vehicle control) but does not affect thermal or mechanical sensitivity in the control (non-inflamed) hindpaw over a multiday time course[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    344.43

    Formula

    C17H20N4O2S
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C(C1=C(NC(C2CCCCC2)=O)SC(NC3=CC=CC=C3)=N1)N
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (362.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9033 mL 14.5167 mL 29.0335 mL
    5 mM 0.5807 mL 2.9033 mL 5.8067 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (6.04 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.9033 mL 14.5167 mL 29.0335 mL 72.5837 mL
    5 mM 0.5807 mL 2.9033 mL 5.8067 mL 14.5167 mL
    10 mM 0.2903 mL 1.4517 mL 2.9033 mL 7.2584 mL
    15 mM 0.1936 mL 0.9678 mL 1.9356 mL 4.8389 mL
    20 mM 0.1452 mL 0.7258 mL 1.4517 mL 3.6292 mL
    25 mM 0.1161 mL 0.5807 mL 1.1613 mL 2.9033 mL
    30 mM 0.0968 mL 0.4839 mL 0.9678 mL 2.4195 mL
    40 mM 0.0726 mL 0.3629 mL 0.7258 mL 1.8146 mL
    50 mM 0.0581 mL 0.2903 mL 0.5807 mL 1.4517 mL
    60 mM 0.0484 mL 0.2419 mL 0.4839 mL 1.2097 mL
    80 mM 0.0363 mL 0.1815 mL 0.3629 mL 0.9073 mL
    100 mM 0.0290 mL 0.1452 mL 0.2903 mL 0.7258 mL
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    UNC3230 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    UNC32301031602-63-7UNC 3230UNC-3230PI4P5KPhosphatidylinositol 4-phosphate 5-kinasesPIP5K1CDRGneuronantinociceptivePIP2PIP4K2CthermalhyperalgesiaglycolysisanticancerInhibitorinhibitorinhibit

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