2. Cell Cycle/DNA Damage
    Apoptosis
  3. DNA/RNA Synthesis
    Apoptosis
  4. YK-4-279

YK-4-279 

Cat. No.: HY-14507 Purity: 99.61%
COA Handling Instructions

YK-4-279 blocks RNA Helicase A (RHA) binding with EWS-FLI1 (oncogenic protein). YK-4-279 induces apoptosis and shows anti-proliferation activities towards various cancer cells. YK-4-279 has a chiral center and it can be separated into two enantiomers. YK-4-279 can be used for the research of cancer.

For research use only. We do not sell to patients.

YK-4-279 Chemical Structure

YK-4-279 Chemical Structure

CAS No. : 1037184-44-3

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Free Sample (0.1 - 0.5 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 117 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 117 In-stock
Solid
10 mg USD 106 In-stock
50 mg USD 407 In-stock
100 mg USD 746 In-stock
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500 mg   Get quote  

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Customer Review

Based on 6 publication(s) in Google Scholar

Top Publications Citing Use of Products

    YK-4-279 purchased from MCE. Usage Cited in: Int J Med Sci. 2017 Apr 7;14(4):356-366.  [Abstract]

    Effects of Docetaxel and YK-4-279 alone or in combination on the levels of ETV1, AR, PSA, p-STAT3, survivin, Bcl-2 and p-Akt in LNCaP and PC-3 cells. LNCaP and PC-3 cells are cultured at a density of 1 × 105 cells/mL in 100 mm tissue culture dishes (10 mL/dish) for 24 h. The cells are then treated with Docetaxel (0.5 nM) and YK-4-279(0.5 µM) alone or in combination for 24 h (for analysis of p-Akt, survivin, PSA, p-STAT3 and AR) and 48 h (for analysis of Bcl-2). The levels of AR,Bcl-2,

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    YK-4-279 blocks RNA Helicase A (RHA) binding with EWS-FLI1 (oncogenic protein). YK-4-279 induces apoptosis and shows anti-proliferation activities towards various cancer cells. YK-4-279 has a chiral center and it can be separated into two enantiomers. YK-4-279 can be used for the research of cancer[1][2].

    IC50 & Target

    IC50: 0.94 µM (TC32), 1.83 µM (TC71), 1.03 µM (RDES), 0.33 µM (SKES), 0.94 µM (MMH-ES-1), 0.60 µM (STA-ET 7.2), 1.46 µM (A4573), 4.95 µM (PC3), 22.82 µM ( MCF7), 0.82 µM (MDA-MB-231), 1.514 µM (PANC1), 14.28 µM (ASPC1)[2]
    In Vitro

    YK-4-279 (3-30 µM; overight) dissociates EWS-FLI1 from RHA in Ewing’s sarcoma family tumor (ESFT) cells[1].
    YK-4-279 (3-30 µM; 14 h) nearly eliminates cyclin D1 in TC32 cells[1].
    YK-4-279 (3-30 µM; 72 h) potently and specifically inhibits ESFTs[1].
    YK-4-279 (50 µM; 6 h) induces substantial apoptosis of ESFT cells[1].
    YK-4-279 (0.1-30 µM; 72 h) inhibits the growth of ESFT, prostate, breast and pancreatic cancer cells[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: TC32, ES925, GUES1, TC71, A673, A4573, CHP100, PANC1, ASP1, MCF-7, MDA-MB-231, PC-3, HFK and HEC cell lines
    Concentration: 3-30 µM
    Incubation Time: 72 h
    Result: Inhibited cell growth with IC50s of 900 nM, 1 μM and 8 μM for TC32, ES925 and GUES1 cells, respectively.

    Apoptosis Analysis[1]

    Cell Line: TC32, HEK, HEC and HFK cell lines
    Concentration: 50 µM
    Incubation Time: 6 h
    Result: Induced apoptosis of ESFT cells and increased caspase-3 activity.

    Cell Viability Assay[2]

    Cell Line: TC32, TC71, RDES, SKES, MMH-ES-1, STA-ET 7.2, A4573, PC3, MCF7, MDA-MB-231, PANC1 and ASPC1cell lines
    Concentration: 0.1-30 µM
    Incubation Time: 72 h
    Result: Inhibited cell growth with IC50s of 0.94, 1.83, 1.03, 0.33, 0.94, 0.60, 1.46, 4.95, 22.82, 0.82, 1.514 and 14.28 µM for TC32, TC71, RDES, SKES, MMH-ES-1, STA-ET 7.2, A4573, PC3, MCF7, MDA-MB-231, PANC1 and ASPC1 cells, respectively.
    In Vivo

    YK-4-279 (1.5 mg; i.p. once) inhibited ESFT tumor growth[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Beige mice with orthotopic ESFT and ESFT xenografts[1]
    Dosage: 1.5 mg
    Administration: Intraperitoneal injection; 1.5 mg once
    Result: Effectively redeced tumor volume of CHP100 and ESFT xenografts (TC71 and CHP100).
    Molecular Weight

    366.20

    Appearance

    Solid

    Formula

    C17H13Cl2NO4
    CAS No.
    SMILES

    ClC1=C2C(C(O)(CC(C3=CC=C(OC)C=C3)=O)C(N2)=O)=C(Cl)C=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (68.27 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7307 mL 13.6537 mL 27.3075 mL
    5 mM 0.5461 mL 2.7307 mL 5.4615 mL
    10 mM 0.2731 mL 1.3654 mL 2.7307 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation
    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Keywords:

    YK-4-2791037184-44-3DNA/RNA SynthesisApoptosisapoptosisanti-proliferationchiralenantiomerESFTprostatebreastpancreaticmiceInhibitorinhibitorinhibit

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