2. Others Metabolic Enzyme/Protease
  3. Fluorescent Dye Glycosidase
  4. 4-Methylumbelliferyl-α-D-Glucopyranoside

4-Methylumbelliferyl-α-D-Glucopyranoside  (Synonyms: 4-Methylumbelliferyl-α-D-Glucose; 4-Methylumbelliferyl-α-D-Glucoside; 4-MU-α-D-Glucopyranoside)

製品番号: HY-137873 純度: 99.99%
COA 取扱説明書 Technical Support

4-Methylumbelliferyl-α-D-Glucopyranoside (4-Methylumbelliferyl-α-D-Glucose) is a fluorescent substrate for α-glucosidase, which releases the fluorescent moiety 4-methylumbelliferyl (4-MU) upon cleavage. 4-MU has pH-dependent fluorescence excitation activity, with excitation wavelengths of 320 nm at low pH (1.97-6.72) and 360 nm at high pH (7.12-10.3), respectively. The emission wavelength of 4-Methylumbelliferyl-α-D-Glucopyranoside increases with decreasing pH, ranging from 445-455 nm. 4-Methylumbelliferyl-α-D-Glucopyranoside can be used as a biomarker for Fabry and Pompe diseases to quantify α-glucosidase activity in infant blood spot samples.

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4-Methylumbelliferyl-α-D-Glucopyranoside

4-Methylumbelliferyl-α-D-Glucopyranoside 構造式

CAS 番号 : 17833-43-1

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Solid
5 mg $25 在庫あり
10 mg $40 在庫あり
25 mg $80 在庫あり
50 mg $118 在庫あり
100 mg $178 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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4-Methylumbelliferyl-α-D-Glucopyranoside 関連抗体

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製品説明

4-Methylumbelliferyl-α-D-Glucopyranoside (4-Methylumbelliferyl-α-D-Glucose) is a fluorescent substrate for α-glucosidase, which releases the fluorescent moiety 4-methylumbelliferyl (4-MU) upon cleavage. 4-MU has pH-dependent fluorescence excitation activity, with excitation wavelengths of 320 nm at low pH (1.97-6.72) and 360 nm at high pH (7.12-10.3), respectively. The emission wavelength of 4-Methylumbelliferyl-α-D-Glucopyranoside increases with decreasing pH, ranging from 445-455 nm. 4-Methylumbelliferyl-α-D-Glucopyranoside can be used as a biomarker for Fabry and Pompe diseases to quantify α-glucosidase activity in infant blood spot samples.

体外実験

Guide (The following is our recommended protocol. This protocol is provided as a guide only and should be modified to suit your specific needs).
GAA enzyme assay[2]
1. Preparation of 4-Methylumbelliferyl-α-D-Glucopyranoside working solution
1) Prepare 2 mM 4-Methylumbelliferyl-α-D-Glucopyranoside working solution.
2. Experimental steps
1) Harvest fibroblasts and HEK293T cells by trypsinization and disrupt by freeze-thawing (3 times).
2) Mix the cell homogenate (30-40 μg protein) with 2 mM 4-Methylumbelliferyl-α-D-Glucopyranoside in 0.2 acetate buffer (pH 4.0) and incubate at 37° for 60 min.
3) The reaction is stopped with 1.9 mL of glycine carbonate buffer (pH 10.7) and fluorescence is read on a Turner Biosystems Modulus 9200 fluorometer (360 nm excitation, 450 nm emission).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Methylumbelliferyl-α-D-Glucopyranoside 関連抗体
分子量

338.31

分子式

C16H18O8
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(C)C2=CC=C(O[C@@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)C=C2O1
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
溶剤 & 溶解度
体外: 

DMSO : 25 mg/mL (73.90 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9559 mL 14.7793 mL 29.5587 mL
5 mM 0.5912 mL 2.9559 mL 5.9117 mL
10 mM 0.2956 mL 1.4779 mL 2.9559 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

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In Vivo Dissolution Calculator
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純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9559 mL 14.7793 mL 29.5587 mL 73.8967 mL
5 mM 0.5912 mL 2.9559 mL 5.9117 mL 14.7793 mL
10 mM 0.2956 mL 1.4779 mL 2.9559 mL 7.3897 mL
15 mM 0.1971 mL 0.9853 mL 1.9706 mL 4.9264 mL
20 mM 0.1478 mL 0.7390 mL 1.4779 mL 3.6948 mL
25 mM 0.1182 mL 0.5912 mL 1.1823 mL 2.9559 mL
30 mM 0.0985 mL 0.4926 mL 0.9853 mL 2.4632 mL
40 mM 0.0739 mL 0.3695 mL 0.7390 mL 1.8474 mL
50 mM 0.0591 mL 0.2956 mL 0.5912 mL 1.4779 mL
60 mM 0.0493 mL 0.2463 mL 0.4926 mL 1.2316 mL
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4-Methylumbelliferyl-α-D-Glucopyranoside Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Methylumbelliferyl-α-D-Glucopyranoside17833-43-14-Methylumbelliferyl-α-D-Glucose 4-Methylumbelliferyl-α-D-Glucoside 4-MU-α-D-GlucopyranosideFluorescent DyeGlycosidaseInhibitorinhibitorinhibit

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4-Methylumbelliferyl-α-D-Glucopyranoside
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