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  4. 5(6)-FAM SE

5(6)-FAM SE  (Synonyms: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester; 5(6)-Carboxyfluorescein succinimidyl ester mixed isomers)

Art. -Nr.: HY-15937 Reinheit: 95.76%
COA
SDS
Handling Instructions Technical Support

5(6)-FAM SE is a green fluorescent dye widely used for protein labeling.

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5(6)-FAM SE

5(6)-FAM SE Chemische Struktur

CAS. Nr. : 117548-22-8

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Based on 4 publication(s) in Google Scholar

5(6)-FAM SE Related Antibodies

Top Publications Citing Use of Products

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Beschreibung

5(6)-FAM SE is a green fluorescent dye widely used for protein labeling.

In Vitro

5(6)-FAM SE labels proteins, which decreases exponentially during the cell division, and corresponds to the cell proliferation degree. 5(6)-FAM SE can be used to assess the cell proliferation degree and cell cycle status[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5(6)-FAM SE Related Antibodies
Molekulargewicht

473.39

Formel

C25H15NO9
CAS. Nr.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

515
Excitation (Ex)

488
SMILES

OC1=CC=C2C(OC(C(C=C3)=C2C(C=CC(C(ON(C(CC4)=O)C4=O)=O)=C5)=C5C(O)=O)=CC3=O)=C1.OC6=CC=C7C(OC(C(C=C8)=C7C(C=C(C(ON(C(CC9)=O)C9=O)=O)C=C%10)=C%10C(O)=O)=CC8=O)=C6
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 350 mg/mL (739.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1124 mL 10.5621 mL 21.1242 mL
5 mM 0.4225 mL 2.1124 mL 4.2248 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Reinheit & Dokumentation

Purity: 95.76%

SDS (393 KB)

COA (256 KB) HNMR (311 KB) LCMS (89 KB)

Handling Instructions (2659 KB)
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1124 mL 10.5621 mL 21.1242 mL 52.8106 mL
5 mM 0.4225 mL 2.1124 mL 4.2248 mL 10.5621 mL
10 mM 0.2112 mL 1.0562 mL 2.1124 mL 5.2811 mL
15 mM 0.1408 mL 0.7041 mL 1.4083 mL 3.5207 mL
20 mM 0.1056 mL 0.5281 mL 1.0562 mL 2.6405 mL
25 mM 0.0845 mL 0.4225 mL 0.8450 mL 2.1124 mL
30 mM 0.0704 mL 0.3521 mL 0.7041 mL 1.7604 mL
40 mM 0.0528 mL 0.2641 mL 0.5281 mL 1.3203 mL
50 mM 0.0422 mL 0.2112 mL 0.4225 mL 1.0562 mL
60 mM 0.0352 mL 0.1760 mL 0.3521 mL 0.8802 mL
80 mM 0.0264 mL 0.1320 mL 0.2641 mL 0.6601 mL
100 mM 0.0211 mL 0.1056 mL 0.2112 mL 0.5281 mL
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5(6)-FAM SE Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5(6)-FAM SE117548-22-85(6)-Carboxyfluorescein N-hydroxysuccinimide ester5(6)-Carboxyfluorescein succinimidyl ester mixed isomersFluorescent DyeInhibitorinhibitorinhibit

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