8-Oxo-dGTP trisodium solution (100 mM)
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8-Oxo-dGTP (8-Oxo-Deoxyguanosine triphosphate) trisodium solution (100mM) is an oxidized guanine nucleotide formed by ROS-mediated oxidative modification of dGTP, and it also serves as a key substrate for 8-oxo-dGTP pyrophosphohydrolases (such as hMTH1 and E. coli MutT). 8-Oxo-dGTP trisodium solution (100mM) acts as a DNA mutagen, inserts into nascent DNA and pairs with adenine and cytosine, inducing A:T to C:G transversion mutations. Furthermore, 8-Oxo-dGTP trisodium solution (100mM) causes oxidative DNA base modification, strand breakage and S-phase arrest, and ultimately triggers AIF-mediated apoptosis and promotes spontaneous carcinogenesis in mth1-deficient mice. Accumulation of 8-Oxo-dGTP trisodium solution (100mM) in cells induces genomic instability, but it exhibits a tumor-suppressive effect that reduces tumor incidence in mouse models instead. 8-Oxo-dGTP trisodium solution (100mM) is widely used in studies related to spontaneous carcinogenesis, Parkinson's disease, Alzheimer's disease, heart failure and tumor mechanisms.
For research use only. We do not sell to patients.
- Purity: 98.95%
- Formula: C10H13N5Na3O14P3
- Molecular Weight:589.13
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Storage:
Solution, -20°C, 2 years
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
8-Oxo-dGTP trisodium solution (100mM) (100 μM) binds to wild-type hMTH1 with high affinity (Kd=0.08 μM), while the F27A and D119A mutations reduce the binding affinity, and the W117A mutation completely abolishes the binding activity[1].
8-Oxo-dGTP trisodium solution (100mM) (2-8 mM; 24 h) inhibits the viability of HeLa-shGFP and HeLa-shMTH1 cells in a dose-dependent manner, with the inhibitory effects at concentrations of 6 mM and 8 mM being stronger than that of 50 μg/mL 5-FU[4].
8-Oxo-dGTP trisodium solution (100mM) (2 mM, 6 mM; 8-24 h) increases the level of 8-oxo-dG in DNA of HeLa-shGFP cells in a dose- and time-dependent manner, and this elevation reaches comparable levels in HeLa cells with MTH1, OGG1 or MUTYH knockdown after 24 h of treatment[4].
8-Oxo-dGTP trisodium solution (100mM) (6 mM; 24 h) induces S-phase cell cycle arrest in HeLa-shGFP and HeLa-shMTH1 cells, with a more pronounced effect in MTH1-knockdown HeLa cells; treatment with 6 mM 8-oxo-dGTP for 12 h also induces S-phase arrest in double thymidine-synchronized HeLa-shGFP and HeLa-shMTH1 cells[4].
8-Oxo-dGTP trisodium solution (100mM) (2 mM, 6 mM; 24 h) induces apoptosis in HeLa-shGFP, HeLa-shMTH1, HeLa-shOGG1 and HeLa-shMUTYH cells via an AIF-mediated caspase-independent pathway, which is evidenced by elevated levels of cleaved PARP and nuclear translocation of AIF[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
8-oxo-dGTP (0.5 mg/kg; intravenous injection; once every 3 days; for 32 consecutive days) trisodium inhibits the growth of subcutaneous HeLa-shMTH1 xenografts in nude mice[4].
8-oxo-dGTP (0.5-2.5 mg/kg; intravenous injection; once every 5 days; for 112 consecutive days) trisodium dose-dependently inhibits spontaneous intestinal adenoma formation[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Liquid
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Molecular Weight 589.13
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Formula C10H13N5Na3O14P3
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Color Colorless to light yellow
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SMILES
O[C@H]1C[C@H](N2C(N=C(N)NC3=O)=C3N=C2O)O[C@@H]1COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O
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Synonyms
8-Oxo-Deoxyguanosine triphosphate trisodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, 2 years
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Sakai Y, et al. A molecular basis for the selective recognition of 2-hydroxy-dATP and 8-oxo-dGTP by human MTH1. J Biol Chem. 2002;277(10):8579-8587. [Content Brief]
[3]. Tsutsui H, et al. 8-oxo-dGTPase, which prevents oxidative stress-induced DNA damage, increases in the mitochondria from failing hearts. Circulation. 2001;104(24):2883-2885. [Content Brief]
[4]. Li J, et al. 8-oxo-dGTP curbs tumor development via S phase arrest and AIF-mediated apoptosis. Free Radic Biol Med. 2023;196:53-64. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
8-Oxo-dGTP trisodium solution (100 mM) Related Classifications
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