Copy Link Print Information

A-485

110 Cited Publications
Customer Review

Based on 110 publication(s) in Google Scholar

A-485, a chemical probe, is a potent and selective catalytic inhibitor of p300/CBP with IC50s of 9.8 nM and 2.6 nM for p300 and CBP histone acetyltransferase (HAT), respectively.

For research use only. We do not sell to patients.

  • Purity: 99.87%
  • CAS No.: 1889279-16-6
  • Formula: C25H24F4N4O5
  • Molecular Weight:536.48
  • Storage:
    Powder   -20°C, 3 years , 4°C, 2 years ; In solvent   -80°C, 2 years , -20°C, 1 year
  • A-485 Chemical Structure
    A-485 Chemical Structure
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
  • Experimental Validation Results for A-485
CAS No.: 1889279-16-6
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Data Download
Other Forms of A-485:

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Chemical Information
  • Solvent & Solubility
  • Protocol
  • Purity & Documentation
  • References
  • Help & FAQs
You May Still Need
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) A-485

More
Customer Validation & Images
WBCell Proliferation/Viability AssayIFIHCCell Migration/Invasion AssayRT-PCR
  • Cell Proliferation/Viability Assay
  • WB
  • WB
  • RT-PCR
  • IF
  • IHC
  • WB
  • RT-PCR
  • RT-PCR
  • Cell Migration/Invasion Assay
  • WB
  • RT-PCR
  • RT-PCR
  • WB
  • WB

All Epigenetic Reader Domain Isoforms

More

All Histone Acetyltransferase Isoforms

More
Keywords
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00
Sign in to MedChemExpress Sign In
Register
Sign in to MedChemExpress
Register New Account

At least 6 characters

Personalize your experience, you are currently not signed in. For a better experience, please create an online profile, you can:

Obtain points redemption prizes
Check out faster
Add multiple shipping locations
View orders
Assay Types
  • A-485 Chemical Structure
    A-485 Chemical Structure
    1/16
  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Cell. 2025 May 29;188(11):2992-3012.e16. [Abstract]

    Customer Validation
     Human monocytes were stimulated with BCG, LPS (1 ng/mL or indicated concentrations), or cultured in medium (RPMI) ± the indicated concentrations of A-485 (p300i, 1 μM) for 24 h. An MTT assay is used to assess the cellular viability (n = 6 donors, mean ± SEM).

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Aug 1;85(15):2838-2857. [Abstract]

    Customer Validation
     A-485 (2 μM; 24 h) suppresses the ascending tendency of GAPDH K251-Su (0.5-6 μM; 24 h) induced by glutamine in NSCLC cells.

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 29;16(1):8071. [Abstract]

    Customer Validation
     A-485 (0.5-8 μM; 24 h) can partially reverse the lysine acetylation induced by wild-type MCP-p300Core.

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 16;16(1):710. [Abstract]

    Customer Validation
     A-485 (2.6 and 9.8 nM; 8.5 h) reduces the expression of TRIM43 in HFF1 cells infected with HSV-1 (HSV-1 GFP, MOI 3) (8 h), while the expression of ICP0 and ICP27 remains unchanged.

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May;12(20):e2413709. [Abstract]

    Customer Validation
     Immunofluorescence staining of Collagen II and Aggrecan in chondrocytes with A485 (0,5,10,20 μM) treatment in the presence of IL-1β. Scale bar, 25 μm.

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May;12(20):e2413709. [Abstract]

    Customer Validation
     IHC of Collagen II, Aggrecan, MMP3 and MMP13 of articular cartilage in sham, DMM induced OA mice with A485 (articular injections of 2 µL, 20 mM at 1, 3, 5, and 7 W) or DMSO. (n=8 per group). Scale bars, 50 μm.

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Nature. 2023 Oct;622(7981):173-179. [Abstract]

    Customer Validation
     Representative western blots of two biological replicates of HEK293T histones treated with A-485 and NU9056 (10 μM for 24 hours)

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Nature. 2023 Oct;622(7981):173-179. [Abstract]

    Customer Validation
     Kacme (top) and H3K27ac (bottom) ChIP-qPCR in HEK293T cells after A-485 treatment (10 μM for 24 hours).

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Nature. 2023 Oct;622(7981):173-179. [Abstract]

    Customer Validation
     qPCR results showing relative expression levels of indicated transcripts in HEK293T cells after A-485 treatment (10 μM for 24 hours).

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Nature. 2023 Oct;622(7981):173-179. [Abstract]

    Customer Validation
     Quantifications of Transwell assays in indicated PANC-1 cells treated with different concentrations of A-485 (1.25-5 μM)

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]

    Customer Validation
     A485 (10 μM; 24 h) leads to the downregulation of H3K9bhb and CPS1 in BHB-treated CD8+ TM cells; however, the use of 3-TYP (10 μM; 24 h) or Trichostatin A (TSA; 5 nM; 24 h) result in the increase of H3K9bhb and CPS1.

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]

    Customer Validation
     TM cells (IL-15-derived) were treated with 3TY (10 μM), TSA (5 nM), A-485 (10 μM) or BHB (5 mM) for 24 h, then Cps1 mRNA was detected by qPCR.

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jul 14;14(1):4129. [Abstract]

    Customer Validation
     A-485 is a potent Histone acetyltransferase (HAT) inhibitor. Quantification analysis using RT-qPCR shows that Rax and Six3 expression in day 5 cultures are significantly reduced by the addition of A-485 (2 µM) from day 4. Pan-neural marker Sox2 does not show significant change.

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Apr:160:114402. [Abstract]

    Customer Validation
     A485 (1, 3 µM; 4 h) reduces endogenous acetylation of NPM1 in HA-NPM1-transfected Huh7/SR NPM1 KD cells.

  • 1/16

    A-485 purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2019 Dec 9;36(6):630-644.e9. [Abstract]

    Customer Validation
     Western blot of MYC in PDX (11a) cells after ex vivo treatment with A-485 (0.1-1 μM) for 6 h.

  • A-485