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  4. Bovine Serum Albumin

Bovine Serum Albumin  (Synonyms: Albumin bovine serum; BSA)

Cat. No.: HY-D0842 Purity: 98.2%
COA
SDS
Handling Instructions Technical Support

Bovine Serum Albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. BSA is a globular protein that is used in numerous biochemical applications.

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Bovine Serum Albumin

Bovine Serum Albumin 화학구조

CAS No. : 9048-46-8

사이즈 가격 재고 수량
500 mg 해외재고보유
1 g 해외재고보유
5 g 해외재고보유
10 g 해외재고보유
50 g   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 53 publication(s) in Google Scholar

Bovine Serum Albumin Related Antibodies

Top Publications Citing Use of Products

53 Publications Citing Use of MCE Bovine Serum Albumin

  • Biological Activity

  • Protocol

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

Bovine Serum Albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. BSA is a globular protein that is used in numerous biochemical applications.

In Vitro

Bovine serum albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. Bovine serum albumin constitutes ca. 60% of all plasma protein and binds and transports a large number of physiological and non-physiological ligands. Bovine serum albumin contains 17 disulfide bonds and one unpaired cysteine (Cys34), which facilitates dimerization and also influences higher-order association, since the rate of aggregation is slowed down if Cys34 is covalently bound to another compound. MTT assay for fibril cytotoxicity shows Bovine Serum Albumin is beneficial for cell growth irrespective of its aggregated state. Lack of cytotoxicity is confirmed by membrane permeabilization assays. In the subsequent 40 h, an increase in the viability of the treated cells of 300-400% is observed, indicating that the cells incubated with Bovine Serum Albumin achieve a higher viability than the untreated cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bovine Serum Albumin Related Antibodies
Clinical Trial
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

S=B[*]
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.
용액&용해도
In Vitro: 

H2O : 50 mg/mL (Need ultrasonic)

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL; Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
순도&문서

Purity: 98.2%

SDS (557 KB)

COA (231 KB)

Handling Instructions (2659 KB)
References
Cell Assay 
[1]

Samples are prepared in 96-well microplates and all wells without samples are filled with 200 μL PBS to minimize evaporation. To each well is loaded 100 μL SH-SY5Y growth medium with or without 10.000 SH-SY5Y neuroblastoma cells. The assay is started by adding 25 μL buffer, with or without 5 mg/mL sonicated Bovine Serum Albumin previously incubated for 0, 2 or 96 h at 70°C (giving a final BSA concentration of 1 mg/mL). All sample combinations are loaded in triplets, and for each of the six Bovine Serum Albumin combinations a corresponding triplet is loaded without cells, to be used as blank. One triplet is also loaded without both cells and BSA and one only without Bovine Serum Albumin[1].

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References
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Bovine Serum Albumin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bovine Serum Albumin9048-46-8Albumin bovine serum BSABiochemical Assay ReagentsInhibitorinhibitorinhibit

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상품명:
Bovine Serum Albumin
Cat. No.:
HY-D0842
수량:
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