1. GPCR/G Protein
    Neuronal Signaling
  2. Opioid Receptor

DAMGO 

Cat. No.: HY-P0210 Purity: 98.10%
Handling Instructions

DAMGO is a μ-opioid receptor (μ-OPR ) selective agonist.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

DAMGO Chemical Structure

DAMGO Chemical Structure

CAS No. : 78123-71-4

Size Price Stock Quantity
10 mM * 1 mL USD 102 In-stock
Estimated Time of Arrival: December 31
1 mg USD 50 In-stock
Estimated Time of Arrival: December 31
5 mg USD 90 In-stock
Estimated Time of Arrival: December 31
10 mg USD 160 In-stock
Estimated Time of Arrival: December 31
25 mg USD 340 In-stock
Estimated Time of Arrival: December 31
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

DAMGO is a μ-opioid receptor (μ-OPR ) selective agonist.

IC50 & Target

Kd: 3.46±0.84 nM (native μ-OPR)[1]

In Vitro

DAMGO, a μ-opioid receptor selective agonist, distinguishes between μ- and δ-opioid receptors around their first extracellular loops. In native μ-OPR, the Kd value for DAMGO is 3.46± 0.84 nM (n=3). The chimeric receptor MMDD, in which the carboxy-terminal half of μ-OPR is replaced with the corresponding region of δ-OPR, exhibits an equivalent affinity (Kd=2.13±0.40 nM; n=3) to DAMGO compared with the native μ-OPR[1]. DAMGO is a selective μ-opioid peptide. DAMGO abolishes the neuroprotective effect of CXCL12 in N-methyl-d-aspartate (NMDA) neurotoxicity studies. Regulation of neuronal response to CXCL12 is essential for shaping of developing and mature central nervous system (CNS). To establish whether DAMGO alter the effect of CXCL12 on neuronal survival, the ability of CXCL12 to protect neurons from N-methyl-d-aspartate (NMDA)-induced death is examined in the presence and absence of DAMGO. Cortical cultures are treated with DAMGO (1 and 10 μM). Neurons are subsequently exposed to NMDA (20 min) and/or CXCL12 (added 10 min before NMDA) in the absence of glia and then returned to the original culture dishes with the glial feeder layer. Neuronal death is evaluated after 24 h. DAMGO inhibits neuronal survival promoted by CXCL12[2].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (243.38 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9471 mL 9.7354 mL 19.4708 mL
5 mM 0.3894 mL 1.9471 mL 3.8942 mL
10 mM 0.1947 mL 0.9735 mL 1.9471 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[2]

Neurons (9 days in vitro) are treated with DAMGO (10 μM) for 24 h in their original culture dish, subsequently transferred to a dish containing Mg2+-free saline with glycine (15 μM), and exposed to NMDA (100 μM) and/or CXCL12 (20 nM) in absence of glia. After treatments, neurons are moved back into the original culture dishes containing glia. Neuronal death is evaluated after 24 h. Hoechst 33342 (3 μg/mL) combined with cleaved caspase-3 (1:100) staining is used to identify normal and apoptotic cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

513.59

Formula

C₂₆H₃₅N₅O₆

CAS No.

78123-71-4

Storage
Protect from light
Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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DAMGO
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HY-P0210
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DAMGO

Cat. No.: HY-P0210