Cat. No.: HY-12660

Copy Link Print Information

MPI-0479605

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

MPI-0479605 is a potent and selective ATP-competitive inhibitor of Mps1, with an IC₅₀ of 1.8 nM.

For research use only. We do not sell to patients.

Purity: 99.54%
CAS No.: 1246529-32-7
Formula: C₂₂H₂₉N₇O
Molecular Weight:407.51

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

MPI-0479605 Chemical Structure

CAS No.: 1246529-32-7

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

MPI-0479605 is a potent and selective ATP-competitive inhibitor of Mps1, with an IC₅₀ of 1.8 nM.

IC₅₀ & Target^[1]

Mps1

1.8 nM (IC₅₀)

ALK

0.26 μM (IC₅₀)

B-RAF

3.2 μM (IC₅₀)

ERK2

3.9 μM (IC₅₀)

FAK1

2.7 μM (IC₅₀)

FER

0.59 μM (IC₅₀)

FLT3

0.08 μM (IC₅₀)

INSR

0.38 μM (IC₅₀)

JNK1

0.11 μM (IC₅₀)

PLK4

3.3 μM (IC₅₀)

STK33

1.1 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>10 μM Compound: 2, MPI-0479605	Antiproliferative activity against human A549 cells after 72 hrs by WST8 assay Antiproliferative activity against human A549 cells after 72 hrs by WST8 assay	[PMID: 23634759]
DLD-1	IC₅₀	>2000 nM Compound: Chemical probe: MPI- 0479605	Inhibition of colony formation in human DLD-1 cells harboring Mps1 S611R mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human DLD-1 cells harboring Mps1 S611R mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
DLD-1	IC₅₀	183 nM Compound: Chemical probe: MPI- 0479605	Inhibition of colony formation in human DLD-1 cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human DLD-1 cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
DLD-1	IC₅₀	75.4 nM Compound: Chemical probe: MPI- 0479605	Antiproliferative activity against human DLD-1 cells assessed as reduction in cell viability incubated for 5 days Antiproliferative activity against human DLD-1 cells assessed as reduction in cell viability incubated for 5 days	[PMID: 26364596]
HCT-116	EC₅₀	0.3 μM Compound: 1, MPI-0479605	Cell cycle arrest in human HCT116 cells assessed as G2 checkpoint inhibition by Hoescht 33342 staining method Cell cycle arrest in human HCT116 cells assessed as G2 checkpoint inhibition by Hoescht 33342 staining method	[PMID: 22632936]
HCT-116	IC₅₀	>2000 nM Compound: Chemical probe: MPI- 0479605	Inhibition of colony formation in human HCT-116 cells harboring Mps1 C604Y mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HCT-116 cells harboring Mps1 C604Y mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HCT-116	IC₅₀	0.023 μM Compound: 1a	Cytotoxicity against human HCT116 cells Cytotoxicity against human HCT116 cells	[PMID: 24183538]
HCT-116	IC₅₀	0.1 μM Compound: 1, MPI-0479605	Cytotoxicity against human HCT116 cells Cytotoxicity against human HCT116 cells	[PMID: 22632936]
HCT-116	IC₅₀	1079 nM Compound: Chemical probe: MPI- 0479605	Inhibition of colony formation in human HCT-116 cells harboring Mps1 I531M mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HCT-116 cells harboring Mps1 I531M mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HCT-116	IC₅₀	159 nM Compound: Chemical probe: MPI- 0479605	Inhibition of colony formation in human HCT-116 cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HCT-116 cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HCT-116	IC₅₀	63 nM Compound: Chemical probe: MPI- 0479605	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 5 days Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 5 days	[PMID: 26364596]
HeLa	IC₅₀	>2000 nM Compound: Chemical probe: MPI- 0479605	Inhibition of colony formation in human HeLa cells harboring Mps1 C604F mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HeLa cells harboring Mps1 C604F mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HeLa	IC₅₀	>2000 nM Compound: Chemical probe: MPI- 0479605	Inhibition of colony formation in human HeLa cells harboring Mps1 C604Y mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HeLa cells harboring Mps1 C604Y mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HeLa	IC₅₀	282 nM Compound: Chemical probe: MPI- 0479605	Inhibition of colony formation in human HeLa cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HeLa cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HeLa	IC₅₀	468 nM Compound: Chemical probe: MPI- 0479605	Inhibition of colony formation in human HeLa cells harboring Mps1 I598F mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HeLa cells harboring Mps1 I598F mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
U2OS	IC₅₀	105.6 nM Compound: Chemical probe: MPI- 0479605	Antiproliferative activity against human U2OS cells assessed as reduction in cell viability incubated for 5 days Antiproliferative activity against human U2OS cells assessed as reduction in cell viability incubated for 5 days	[PMID: 26364596]

In Vitro

MPI-0479605 is a potent and selective ATP-competitive inhibitor of Mps1, with an IC₅₀ of 1.8 nM. MPI-0479605 (0.1-10 μM) reduces cell viability of HCT-116 cells in a dose-dependent manner. MPI-0479605 shows severe defects in the ability to align chromosomes at the metaphase plate, but causes complete cytokinesis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

MPI-0479605 (30 mg/kg daily or 150 mg/kg every fourth day (Q4D), i.p.) inhibits tumor growth by 49% and 74 % in HCT-116 xenografts. However, MPI-0479605 does not show inhibitory activity via daily dosing on the Colo-205 xenografts, and dosing every four days causes 63% tumor growth inhibition (TGI)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1246529-32-7
Appearance Solid
Molecular Weight 407.51
Formula C₂₂H₂₉N₇O
Color White to gray
SMILES

CC(C=C(N1CCOCC1)C=C2)=C2NC3=NC4=C(N=CN4)C(NC5CCCCC5)=N3
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (7)

All

Sort by:

Journal Impact Factor

Nature

Evolutionary fingerprints of epithelial-to-mesenchymal transition. [Abstract]

2025 Apr;640(8060):1083-1092. PMID: 40044861
Nature

Aneuploidy renders cancer cells vulnerable to mitotic checkpoint inhibition. [Abstract]

2021 Feb;590(7846):486-491. PMID: 33505028
Nat Cell Biol

Chromosome mis-segregation triggers cell cycle arrest through a mechanosensitive nuclear envelope checkpoint. [Abstract]

2025 Jan;27(1):73-86. PMID: 39779939
Breast Cancer Res Treat

Monopolar spindle 1 contributes to tamoxifen resistance in breast cancer through phosphorylation of estrogen receptor α. [Abstract]

2023 Dec;202(3):595-606. PMID: 37695401
bioRxiv

2026 Apr 29.
University of Califomia San Francisco

2026.
bioRxiv

2020 Jun.

Solvent & Solubility

In Vitro:

DMSO : 11 mg/mL (26.99 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4539 mL

12.2696 mL

24.5393 mL

5 mM

0.4908 mL

2.4539 mL

4.9079 mL

10 mM

0.2454 mL

1.2270 mL

2.4539 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Protocol

Animal Administration^[1]

HCT-116 or Colo-205 cells are transplanted subcutaneously into the flanks of nude (nu⁺/nu⁺) mice and compound (MPI-0479605) treatment is initiated when tumor masses reaches an average size of 100 mm³. MPI-0479605 is formulated in 5% dimethylacetamide (DMA)/12% ethanol/40% PEG-300, ispinesib is formulated in 2% cremaphor/2% DMA, and 5-fluorouracil is formulated in 2% sodium bicarbonate. Tumor volume is measured using vernier calipers and tumor growth inhibition (TGI) is calculated as: %TGI= 100-100(change in median tumor volume of treated)/(change in median tumor volume control)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tardif KD, et al. Characterization of the cellular and antitumor effects of MPI-0479605, a small-molecule inhibitor of the mitotic kinase Mps1. Mol Cancer Ther. 2011 Dec;10(12):2267-2275. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4539 mL	12.2696 mL	24.5393 mL	61.3482 mL
	5 mM	0.4908 mL	2.4539 mL	4.9079 mL	12.2696 mL
	10 mM	0.2454 mL	1.2270 mL	2.4539 mL	6.1348 mL
	15 mM	0.1636 mL	0.8180 mL	1.6360 mL	4.0899 mL
	20 mM	0.1227 mL	0.6135 mL	1.2270 mL	3.0674 mL
	25 mM	0.0982 mL	0.4908 mL	0.9816 mL	2.4539 mL

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

MPI-0479605

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Kinase Inhibitor Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Cytoskeleton Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Multi-Target Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) MPI-0479605

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

MPI-0479605

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Kinase Inhibitor Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Cytoskeleton Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Multi-Target Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) MPI-0479605

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

MPI-0479605 Related Classifications

HY-12660 Related Classifications