NADPH tetrasodium salt

Name: NADPH tetrasodium salt
Brand: MedChemExpress
SKU: HY-F0003
Rating: 5.0 (18 reviews)

Cat. No.: HY-F0003 Purity: 99.98%: Data Sheet
COA

SDS
Handling Instructions
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NADPH tetrasodium salt functions as an important cofactor in a variety of metabolic and biosynthetic pathways. NADPH tetrasodium salt is an endogenous inhibitor of ferroptosis. NADPH tetrasodium salt plays a vital role in the biosynthesis of agents, chiral alcohols, fatty acids and biopolymers, while also being required for lipid biosynthesis, biomass formation, and cell replication.

For research use only. We do not sell to patients.

CAS No. : 2646-71-1

Size	Stock	Quantity
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 18 publication(s) in Google Scholar

Other Forms of NADPH tetrasodium salt:

NADPH In-stock
NADPH tetracyclohexanamine In-stock
NADPH tetrasodium salt (Standard) In-stock

18 Publications Citing Use of MCE NADPH tetrasodium salt

Cell Imaging/Staining

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

: NADPH tetrasodium salt purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 15:e07283. [Abstract]; A normal cellular state was recovered by adding TEMPO or NADPH (1mM, 24h), or SLC7A11 knockdown.

: NADPH tetrasodium salt purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 15:e07283. [Abstract]; Western Blot analysis of RAC/WAVE pathway-related indicators in vitro skeletal muscle cells. Muscle cells treated with different condition NADPH(1mM, 24h).

: NADPH tetrasodium salt purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 15:e07283. [Abstract]; mtROS fluorescence staining of skeletal muscle cells in vitro. Muscle cells treated with different condition NADPH(1mM, 24h).

: NADPH tetrasodium salt purchased from MedChemExpress. Usage Cited in: Mol Plant Pathol. 2025 Dec;26(12):e70196. [Abstract]; Enzymatic activities of AtCAD7, AtCAD5, EcYahK, and EcYjgB toward (Z)‐3‐hexenal, acrolein, isovaleraldehyde, salicylaldehyde, and p‐coumaryl aldehyde. No Substrate was negative control. Assays were conducted under standardised conditions (30°C, pH 6.5, 100 μM substrate, 200 μM NADPH).

: NADPH tetrasodium salt purchased from MedChemExpress. Usage Cited in: Mol Ther Oncol. 2024 Sep 17;32(4):200878. [Abstract]; Apoptosis proportion of oncoVV-AVL-treated AsPC-1(Up) and PANC-1 (Down) cells treated with NADPH (300 μM) or NADH. Data are presented as the mean ± SD (n=3).

: NADPH tetrasodium salt purchased from MedChemExpress. Usage Cited in: Mol Ther Oncol. 2024 Sep 17;32(4):200878. [Abstract]; Western blotting detection of the expressions of caspase-3 and caspase-9 in oncoVV or oncoVV-AVL-treated AsPC-1(Up) and PANC-1 (Down) cells treated with NADPH (300 μM).

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target^[1]

Human Endogenous Metabolite

In Vitro

The regeneration rate of NADPH tetrasodium salt is often the rate-limiting step for the over-production of desired chemicals, while maintaining robust cellular growth^[2].
NADPH tetrasodium salt homeostasis is regulated by varied signaling pathways and several metabolic enzymes that undergo adaptive alteration in cancer cells. The metabolic reprogramming of NADPH tetrasodium salt renders cancer cells both highly dependent on this metabolic network for antioxidant capacity and more susceptible to oxidative stress^[3].
NADPH tetrasodium salt is an essential intracellular reductant needed to eliminate lipid hydroperoxides. Indeed, NADPH tetrasodium salt levels are a biomarker of ferroptosis sensitivity across many cancer cell lines^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

833.35

Formula

C₂₁H₂₆N₇Na₄O₁₇P₃

CAS No.

2646-71-1

Appearance

Solid

Color

White to light yellow

SMILES

O[C@H]1[C@@H](O)[C@H](N2C=C(C(N)=O)CC=C2)O[C@@H]1COP(OP(OC[C@@H]3[C@@H](O)[C@@H](OP(O[Na])(O[Na])=O)[C@H](N(C4=NC=N5)C=NC4=C5N)O3)(O[Na])=O)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (150.00 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2000 mL	5.9999 mL	11.9998 mL
5 mM	0.2400 mL	1.2000 mL	2.4000 mL
10 mM	0.1200 mL	0.6000 mL	1.2000 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (120.00 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (283 KB) SDS (394 KB)

COA (263 KB) HNMR (317 KB) RP-HPLC (256 KB) MS (71 KB)

Handling Instructions (2659 KB)

References

[1]. Tedeschi PM, et al. NAD+ Kinase as a Therapeutic Target in Cancer. Clin Cancer Res. 2016;22(21):5189-5195. [Content Brief]

[2]. Ng CY, F et al. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metab Eng. 2015;29:86-96. [Content Brief]

[3]. Ju HQ, Lin JF, et al. NADPH homeostasis in cancer: functions, mechanisms and therapeutic implications. Signal Transduct Target Ther. 2020;5(1):231. Published 2020 Oct 7. [Content Brief]

[4]. Stockwell BR, et al. Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease. Cell. 2017;171(2):273-285. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.2000 mL	5.9999 mL	11.9998 mL	29.9994 mL
	5 mM	0.2400 mL	1.2000 mL	2.4000 mL	5.9999 mL
	10 mM	0.1200 mL	0.6000 mL	1.2000 mL	2.9999 mL
	15 mM	0.0800 mL	0.4000 mL	0.8000 mL	2.0000 mL
	20 mM	0.0600 mL	0.3000 mL	0.6000 mL	1.5000 mL
	25 mM	0.0480 mL	0.2400 mL	0.4800 mL	1.2000 mL
	30 mM	0.0400 mL	0.2000 mL	0.4000 mL	1.0000 mL
	40 mM	0.0300 mL	0.1500 mL	0.3000 mL	0.7500 mL
	50 mM	0.0240 mL	0.1200 mL	0.2400 mL	0.6000 mL
	60 mM	0.0200 mL	0.1000 mL	0.2000 mL	0.5000 mL
	80 mM	0.0150 mL	0.0750 mL	0.1500 mL	0.3750 mL
	100 mM	0.0120 mL	0.0600 mL	0.1200 mL	0.3000 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.