Phalloidin (Synonyms: ファロイジン)

製品番号: HY-P0028 純度: 99.83%: Data Sheet SDS COA 取扱説明書
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Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome .

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Phalloidin 構造式

容量	価格（税別）	在庫状況	数量
500 μg	$450	在庫あり	Estimated Time of Arrival: December 31
1 mg	$800	在庫あり	Estimated Time of Arrival: December 31
5 mg	$2000	在庫あり	Estimated Time of Arrival: December 31
10 mg		お問い合わせ
50 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 23 publication(s) in Google Scholar

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製品説明

Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome ^[1].

体外実験

Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

788.87

分子式

C₃₅H₄₈N₈O₁₁S

CAS 番号

17466-45-4

Appearance

Solid

Color

White to off-white

Sequence

Ala-{d-Thr}-Cys-{Hyp}-Ala-Trp-Leu (Sulfide bridge: Cys3-Trp6)

Sequence Shortening

A-{d-Thr}-C-{Hyp}-AWL (Sulfide bridge: Cys3-Trp6)

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

polypeptide, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (polypeptide, sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (126.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2676 mL	6.3382 mL	12.6764 mL
5 mM	0.2535 mL	1.2676 mL	2.5353 mL
10 mM	0.1268 mL	0.6338 mL	1.2676 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

純度とドキュメンテーション

純度: 99.83%

Select Batch:

データシート (270 KB) SDS (420 KB)

COA (242 KB) RP-HPLC (181 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947. [Content Brief]

細胞実験 ^[1]	Prepare a 1:200 dilution of the stock solution (300 units/mL) of fluorescein Phalloidin or rhodamine Phalloidin using PBS. Aspirate the cell medium from the cells grown on glass coverslips and rinse the cells three times with PBS. Fix the cells for 10 min in the 3.7% formaldehyde solution. Rinse the fixed cells three times for 5 min each in PBS. Permeabilize the cells for 5 min in the 0.2% Triton X-100 solution. Rinse the fixed cells three times with PBS. Label the cells with the fluorescein or rhodamine Phalloidin for 5 to 10 min at room temperature. Rinse the cells three times in PBS for 5 min each time. Mount the cell and view the cells using either the fluorescein or rhodamine filter set, depending on the probe^[1]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
参考文献	[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947. [Content Brief]

細胞実験
^[1]

Prepare a 1:200 dilution of the stock solution (300 units/mL) of fluorescein Phalloidin or rhodamine Phalloidin using PBS. Aspirate the cell medium from the cells grown on glass coverslips and rinse the cells three times with PBS. Fix the cells for 10 min in the 3.7% formaldehyde solution. Rinse the fixed cells three times for 5 min each in PBS. Permeabilize the cells for 5 min in the 0.2% Triton X-100 solution. Rinse the fixed cells three times with PBS. Label the cells with the fluorescein or rhodamine Phalloidin for 5 to 10 min at room temperature. Rinse the cells three times in PBS for 5 min each time. Mount the cell and view the cells using either the fluorescein or rhodamine filter set, depending on the probe^[1].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。

参考文献

[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2676 mL	6.3382 mL	12.6764 mL	31.6909 mL
	5 mM	0.2535 mL	1.2676 mL	2.5353 mL	6.3382 mL
	10 mM	0.1268 mL	0.6338 mL	1.2676 mL	3.1691 mL
	15 mM	0.0845 mL	0.4225 mL	0.8451 mL	2.1127 mL
	20 mM	0.0634 mL	0.3169 mL	0.6338 mL	1.5845 mL
	25 mM	0.0507 mL	0.2535 mL	0.5071 mL	1.2676 mL
	30 mM	0.0423 mL	0.2113 mL	0.4225 mL	1.0564 mL
	40 mM	0.0317 mL	0.1585 mL	0.3169 mL	0.7923 mL
	50 mM	0.0254 mL	0.1268 mL	0.2535 mL	0.6338 mL
	60 mM	0.0211 mL	0.1056 mL	0.2113 mL	0.5282 mL
	80 mM	0.0158 mL	0.0792 mL	0.1585 mL	0.3961 mL
	100 mM	0.0127 mL	0.0634 mL	0.1268 mL	0.3169 mL

Phalloidin Related Classifications

Others

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phalloidin17466-45-4Fluorescent DyeInhibitorinhibitorinhibit

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Phalloidin (Synonyms: ファロイジン)

カスタマーレビュー

Phalloidin 関連抗体

顧客検証

おすすめ

•関連小分子:

•関連タンパク質:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

Phalloidin 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Phalloidin Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

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