SR-18292
Based on 66 publication(s) in Google Scholar
SR-18292 is a PPAR gamma coactivator-1α (PGC-1α) inhibitor, which increases PGC-1α acetylation, suppresses gluconeogenic gene expression and reduces glucose production in hepatocytes.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 2095432-55-4
- Formula: C23H30N2O2
- Molecular Weight:366.50
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) SR-18292
More- Signal Transduct Target Ther. 2026 Jan 16;11(1):33. [Abstract]
- Nat Aging. 2025 May;5(5):831-847. [Abstract]
- Nat Commun. 2025 May 8;16(1):4292. [Abstract]
- Nat Commun. 2023 Nov 18;14(1):7527. [Abstract]
- J Adv Res. 2024 Jul:61:65-81. [Abstract]
- Redox Biol. 2023 Jun:62:102709. [Abstract]
- Redox Biol. 2021 Jan:38:101771. [Abstract]
- J Nanobiotechnology. 2024 May 22;22(1):276. [Abstract]
- Phytomedicine. 2023 Jan:108:154545. [Abstract]
- Phytomedicine. 2022 Feb:96:153845. [Abstract]
- J Hazard Mater. 2023 Oct 5:459:132262. [Abstract]
- J Transl Med. 2023 Jul 20;21(1):486. [Abstract]
- Int J Biol Macromol. 2025 Nov 26;337(Pt 1):149292. [Abstract]
- J Headache Pain. 2023 Jun 5;24(1):65. [Abstract]
- Mol Med. 2025 Oct 6;31(1):308. [Abstract]
- Phytother Res. 2026 Jun 18. [Abstract]
- Phytother Res. 2024 Mar;38(3):1345-1357. [Abstract]
- Free Radic Biol Med. 2026 Sep:253:463-477. [Abstract]
- Free Radic Biol Med. 2025 Mar 1:229:312-332. [Abstract]
- Clin Transl Med. 2026 Jul;16(7):e70726. [Abstract]
- Br J Pharmacol. 2025 Sep 15. [Abstract]
- Cell Mol Neurobiol. 2019 Mar;39(2):265-286. [Abstract]
- Fundam Res. 2026 Mar 12.
- Front Immunol. 2019 Nov 14:10:2650. [Abstract]
- J Ethnopharmacol. 2023 May 23:308:116276. [Abstract]
- J Agric Food Chem. 2024 Jul 17;72(28):15740-15754. [Abstract]
- Biochem Pharmacol. 2024 Apr:222:116053. [Abstract]
- Life Sci. 2026 Apr 15:391:124281. [Abstract]
- Inflamm Res. 2022 Sep;71(9):1095-1108. [Abstract]
- Cells. 2023 Apr 6;12(7):1098. [Abstract]
- Nutrients. 2024 Nov 13;16(22):3869. [Abstract]
- Acta Physiol. 2021 Apr;231(4):e13595. [Abstract]
- Int J Mol Sci. 2025 Jun 19;26(12):5910. [Abstract]
- Int Immunopharmacol. 2021 Nov:100:108159. [Abstract]
- Inflammation. 2026 Feb 3;49(1):77. [Abstract]
- Front Pharmacol. 2022 Mar 3;13:834570. [Abstract]
- Front Cell Dev Biol. 2020 Jul 28:8:702. [Abstract]
- J Nutr Biochem. 2025 Sep:143:109954. [Abstract]
- Chem Biol Interact. 2024 Jan 25:388:110850. [Abstract]
- World J Stem Cells. 2021 Dec 26;13(12):1928-1946. [Abstract]
- Sci Rep. 2020 Feb 20;10(1):3078. [Abstract]
- Cell Signal. 2026 Feb:138:112221. [Abstract]
- Cell Signal. 2024 Dec:124:111478. [Abstract]
- J Dairy Sci. 2023 Feb;106(2):1315-1329. [Abstract]
- Cell Signal. 2022 Nov:99:110442. [Abstract]
- Neurochem Res. 2023 May;48(5):1504-1515. [Abstract]
- Neurochem Res. 2023 May;48(5):1580-1595. [Abstract]
- Brain Res Bull. 2021 Oct:175:136-149. [Abstract]
- J Funct Foods. 2023 Nov, 110, 105825.
- J Funct Foods. November 2022, 105291.
- J Funct Foods. August 2022, 105178.
- Cytokine. 2023 May:165:156186. [Abstract]
- Toxicol Appl Pharmacol. 2022 Jan 1:434:115798. [Abstract]
- Reprod Toxicol. 2022 Oct:113:18-29. [Abstract]
- Cardiovasc Toxicol. 2026 May 13;26(5):48. [Abstract]
- PPAR Res. 2023 Mar 27:2023:2523536. [Abstract]
- Food Chem Toxicol. 2023 Jan:171:113513. [Abstract]
- Physiol Behav. 2021 Feb 1:229:113236. [Abstract]
- Connect Tissue Res. 2025 Sep 17:1-13. [Abstract]
- Mol Cell Toxicol. 2026 Apr 26.
- bioRxiv. 2025 Oct 22.
- bioRxiv. 2025 February 08.
- Oxid Med Cell Longev. 2022 Oct 3:2022:1231970. [Abstract]
- Oxid Med Cell Longev. 2022 Jun 20;2022:5905374. [Abstract]
- Biomed Pharmacother. 2021 Dec:144:112331. [Abstract]
- Research Square Preprint. 2021 Jul.
Customer Validation & Images
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Biological Activity
PGC-1α[1]
The transcriptional coactivator PGC-1α plays a pivotal role in energy homeostasis by co-activating transcription factors that regulate fat and glucose metabolism. SR-18292 increases the interaction of PGC-1α with the acetyl transferase GCN5 and reduces co-activation of nuclear hormone receptor HNF4α by PGC-1α. SR-18292 suppresses HNF4α/PGC-1α gluconeogenic transcriptional function. By increasing the interaction of GCN5 with PGC-1α, SR-18292 increases the acetylation of specific PGC-1α lysine residues that might subsequently decrease its gluconeogenic activity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2095432-55-4
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Appearance Solid
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Molecular Weight 366.50
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Formula C23H30N2O2
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Color White to light brown
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SMILES
CC1=CC=C(CN(C(C)(C)C)CC(O)COC2=CC=CC3=C2C=CN3)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (66)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Vertical RAS pathway inhibition in pancreatic cancer drives therapeutically exploitable mitochondrial alterations. [Abstract]2026 Jan 16;11(1):33. PMID: 41545339 -
Nat Aging
Mitochondria-enriched hematopoietic stem cells exhibit elevated self-renewal capabilities, thriving within the context of aged bone marrow. [Abstract]2025 May;5(5):831-847. PMID: 40050412 -
Nat Commun
Metabolic reprogramming driven by Ant2 deficiency augments T Cell function and anti-tumor immunity in mice. [Abstract]2025 May 8;16(1):4292. PMID: 40341170 -
Nat Commun
ZHX2 emerges as a negative regulator of mitochondrial oxidative phosphorylation during acute liver injury. [Abstract]2023 Nov 18;14(1):7527. PMID: 37980429 -
J Adv Res
Triad3A-Mediated K48-Linked ubiquitination and degradation of TLR9 impairs mitochondrial bioenergetics and exacerbates diabetic cardiomyopathy. [Abstract]2024 Jul:61:65-81. PMID: 37625569 -
Redox Biol
Mitochondrial fusion induced by transforming growth factor-β1 serves as a switch that governs the metabolic reprogramming during differentiation of regulatory T cells. [Abstract]2023 Jun:62:102709. PMID: 37116255 -
Redox Biol
2021 Jan:38:101771. PMID: 33189984 -
J Nanobiotechnology
Gouqi-derived nanovesicles (GqDNVs) inhibited dexamethasone-induced muscle atrophy associating with AMPK/SIRT1/PGC1α signaling pathway. [Abstract]2024 May 22;22(1):276. PMID: 38778385 -
Phytomedicine
Xuanfei Baidu formula alleviates impaired mitochondrial dynamics and activated NLRP3 inflammasome by repressing NF-κB and MAPK pathways in LPS-induced ALI and inflammation models. [Abstract]2023 Jan:108:154545. PMID: 36423572 -
Phytomedicine
Isoliquiritigenin-mediated miR-23a-3p inhibition activates PGC-1α to alleviate alcoholic liver injury. [Abstract]2022 Feb:96:153845. PMID: 34785106 -
J Hazard Mater
Daucosterol confers protection against T-2 toxin induced blood-brain barrier toxicity through the PGC-1α-mediated defensive response in vitro and in vivo. [Abstract]2023 Oct 5:459:132262. PMID: 37604032 -
J Transl Med
An Fgr kinase inhibitor attenuates sepsis-associated encephalopathy by ameliorating mitochondrial dysfunction, oxidative stress, and neuroinflammation via the SIRT1/PGC-1α signaling pathway. [Abstract]2023 Jul 20;21(1):486. PMID: 37475042 -
Int J Biol Macromol
PGC1-α drives MFN2-linked mitochondrial fusion aiding glioblastoma cell survival under TMZ stress. [Abstract]2025 Nov 26;337(Pt 1):149292. PMID: 41314582 -
J Headache Pain
SS-31 alleviated nociceptive responses and restored mitochondrial function in a headache mouse model via Sirt3/Pgc-1α positive feedback loop. [Abstract]2023 Jun 5;24(1):65. PMID: 37271805 -
Mol Med
CAMKK2 restored mitochondrial dynamics homeostasis to alleviate pulmonary fibrosis via AMPK/PGC-1α signaling pathway in lung fibroblasts. [Abstract]2025 Oct 6;31(1):308. PMID: 41053564 -
Phytother Res
Poria cocos Polysaccharides Protect Against Contrast-Induced Renal Injury Through Activating CaMKK2/AMPK/PGC-1α Signaling Pathway. [Abstract]2026 Jun 18. PMID: 42312878 -
Phytother Res
Emodin alleviates CRS4-induced mitochondrial damage via activation of the PGC1α signaling. [Abstract]2024 Mar;38(3):1345-1357. PMID: 38198804 -
Free Radic Biol Med
Antiandrogen therapy induces mitochondrial oxidative phosphorylation to promote castration resistance in prostate cancer. [Abstract]2026 Sep:253:463-477. PMID: 42219062 -
Free Radic Biol Med
GTPBP8 mitigates nonalcoholic steatohepatitis (NASH) by depressing hepatic oxidative stress and mitochondrial dysfunction via PGC-1α signaling. [Abstract]2025 Mar 1:229:312-332. PMID: 39341301 -
Clin Transl Med
α7nAChR agonist GTS-21 ameliorates sepsis-induced acute kidney injury via MEF2/PGC-1α/HO-1 axis in mice. [Abstract]2026 Jul;16(7):e70726. PMID: 42333777 -
Br J Pharmacol
Beyond TGFβ inhibition: Chitosan oligosaccharide targets PGC-1α-mediated mitochondrial repair to combat pulmonary fibrosis. [Abstract]2025 Sep 15. PMID: 40955098 -
Cell Mol Neurobiol
MALAT1 Up-Regulator Polydatin Protects Brain Microvascular Integrity and Ameliorates Stroke Through C/EBPβ/MALAT1/CREB/PGC-1α/PPARγ Pathway. [Abstract]2019 Mar;39(2):265-286. PMID: 30607811 -
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Front Immunol
Isovitexin-Mediated Regulation of Microglial Polarization in Lipopolysaccharide-Induced Neuroinflammation via Activation of the CaMKKβ/AMPK-PGC-1α Signaling Axis. [Abstract]2019 Nov 14:10:2650. PMID: 31798583 -
J Ethnopharmacol
Yi-Qi-Jian-Pi formula ameliorates immune function in acute-on-chronic liver failure by upregulating autophagy and mitochondrial biogenesis in CD8+ T lymphocytes. [Abstract]2023 May 23:308:116276. PMID: 36806340
SR-18292 purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2023 May 23:308:116276. [Abstract]
SR-18292 (20 μM; pretreat) decreases the expression of autophagy-associated proteins LC3I/LC3II, Beclin1, PINK1, and Parkin, while increases P62 in Jurkat cells (Fig. I and J).
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J Agric Food Chem
Raspberry Ketone Attenuates Hepatic Fibrogenesis and Inflammation via Regulating the Crosstalk of FXR and PGC-1α Signaling. [Abstract]2024 Jul 17;72(28):15740-15754. PMID: 38970822 -
Biochem Pharmacol
PGC-1α activation ameliorates cancer-induced bone pain via inhibiting apoptosis of GABAergic interneurons. [Abstract]2024 Apr:222:116053. PMID: 38354958 -
Life Sci
Oxytocin ameliorates cardiac hypertrophy by inhibiting mitochondrial dysfunction and pyroptosis via AMPK/PGC-1α /TFAM pathway. [Abstract]2026 Apr 15:391:124281. PMID: 41722768 -
Inflamm Res
Heme oxygenase-1 protects against endotoxin-induced acute lung injury depends on NAD+-mediated mitonuclear communication through PGC1α/PPARγ signaling pathway. [Abstract]2022 Sep;71(9):1095-1108. PMID: 35816227 -
Cells
Glucagon Promotes Gluconeogenesis through the GCGR/PKA/CREB/PGC-1α Pathway in Hepatocytes of the Japanese Flounder Paralichthys olivaceus. [Abstract]2023 Apr 6;12(7):1098. PMID: 37048171 -
Nutrients
Resveratrol and Its Derivatives Diminish Lipid Accumulation in Adipocytes In Vitro-Mechanism of Action and Structure-Activity Relationship. [Abstract]2024 Nov 13;16(22):3869. PMID: 39599655 -
Acta Physiol
High phosphate impairs arterial endothelial function through AMPK-related pathways in mouse resistance arteries. [Abstract]2021 Apr;231(4):e13595. PMID: 33835704 -
Int J Mol Sci
Melatonin Alleviates MBP-Induced Oxidative Stress and Apoptosis in TM3 Cells via the SIRT1/PGC-1α Signaling Pathway. [Abstract]2025 Jun 19;26(12):5910. PMID: 40565373 -
Int Immunopharmacol
Isoliquiritigenin alleviates LPS/ D-GalN-induced acute liver failure by activating the PGC-1α/ Nrf2 pathway to reduce oxidative stress and inflammatory response. [Abstract]2021 Nov:100:108159. PMID: 34555641 -
Inflammation
FTO-dependent m6A Demethylation Activates Mxd1 To Enhance Vitamin D-induced Suppression of Neuroinflammation Via PTEN/AKT/PGC-1α Signaling Pathways in Microglia. [Abstract]2026 Feb 3;49(1):77. PMID: 41632223 -
Front Pharmacol
5-HT1F Receptor Agonist Ameliorates Mechanical Allodynia in Neuropathic Pain via Induction of Mitochondrial Biogenesis and Suppression of Neuroinflammation. [Abstract]2022 Mar 3;13:834570. PMID: 35308244 -
Front Cell Dev Biol
Dihydromyricetin Protects Against Gentamicin-Induced Ototoxicity via PGC-1α/SIRT3 Signaling in vitro. [Abstract]2020 Jul 28:8:702. PMID: 32850822
SR-18292 purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2020 Jul 28:8:702. [Abstract]
Treatment with SR-18292 results in the downregulation of PGC-1α and SIRT3, whereas SIRT3 inhibition by 3-TYP application had no effect on the protein expression of PGC-1α in HEI-OC1 cells. Similar results are obtained in cochlear explants.
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J Nutr Biochem
Gut microbiota regulation by Lactiplantibacillus plantarum SG5 enhances mitochondrial function in Parkinson's disease mice via the GLP-1/PGC-1α pathway. [Abstract]2025 Sep:143:109954. PMID: 40368220 -
Chem Biol Interact
Malvidin promotes PGC-1α/Nrf2 signaling to attenuate the inflammatory response and restore mitochondrial activity in septic acute kidney injury. [Abstract]2024 Jan 25:388:110850. PMID: 38135199 -
World J Stem Cells
Urolithin a alleviates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells through SIRT1/PGC-1α pathway. [Abstract]2021 Dec 26;13(12):1928-1946. PMID: 35069991 -
Sci Rep
NIPA2 regulates osteoblast function by modulating mitophagy in type 2 diabetes osteoporosis. [Abstract]2020 Feb 20;10(1):3078. PMID: 32080264 -
Cell Signal
Idebenone alleviates rotenone-induced mitochondrial dysfunction through mitophagy and mitochondrial biogenesis in muscle cells. [Abstract]2026 Feb:138:112221. PMID: 41205881 -
Cell Signal
Sirt1-mediated deacetylation of PGC-1α alleviated hepatic steatosis in type 2 diabetes mellitus via improving mitochondrial fatty acid oxidation. [Abstract]2024 Dec:124:111478. PMID: 39428026 -
J Dairy Sci
Sirtuin 3 relieves inflammatory responses elicited by lipopolysaccharide via the PGC1α-NFκB pathway in bovine mammary epithelial cells. [Abstract]2023 Feb;106(2):1315-1329. PMID: 36494223 -
Cell Signal
Indole-3-acetic acid improves the hepatic mitochondrial respiration defects by PGC1a up-regulation. [Abstract]2022 Nov:99:110442. PMID: 35988807 -
Neurochem Res
Apelin-13 Improves Cognitive Impairment and Repairs Hippocampal Neuronal Damage by Activating PGC-1α/PPARγ Signaling. [Abstract]2023 May;48(5):1504-1515. PMID: 36512295 -
Neurochem Res
SS31 Confers Cerebral Protection by Reversing Mitochondrial Dysfunction in Early Brain Injury Following Subarachnoid Hemorrhage, via the Nrf2- and PGC-1α-Dependent Pathways. [Abstract]2023 May;48(5):1580-1595. PMID: 36574150 -
Brain Res Bull
DRD1 agonist A-68930 improves mitochondrial dysfunction and cognitive deficits in a streptozotocin-induced mouse model. [Abstract]2021 Oct:175:136-149. PMID: 34284074 -
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Cytokine
The Src1-PGC1α-AP1 complex-dependent secretion of substance P induces inflammation and apoptosis in encephalomyocarditis virus-infected mice. [Abstract]2023 May:165:156186. PMID: 36989655 -
Toxicol Appl Pharmacol
Downregulation of HSPA12A underlies myotoxicity of local anesthetic agent bupivacaine through inhibiting PGC1α-mediated mitochondrial integrity. [Abstract]2022 Jan 1:434:115798. PMID: 34793778 -
Reprod Toxicol
Melatonin attenuates di-(2-ethylhexyl) phthalate-induced apoptosis of human granulosa cells by inhibiting mitochondrial fission. [Abstract]2022 Oct:113:18-29. PMID: 35952901 -
Cardiovasc Toxicol
Quercetin Antagonizes Doxorubicin-Induced Cardiotoxicity via HO-1/PGC-1α-ALOX5 Axis: Arachidonic Acid Metabolism-Ferroptosis Crosstalk as a Therapeutic Target. [Abstract]2026 May 13;26(5):48. PMID: 42126728 -
PPAR Res
PPAR- γ Activation Alleviates Osteoarthritis through Both the Nrf2/NLRP3 and PGC-1 α/ Δψ m Pathways by Inhibiting Pyroptosis. [Abstract]2023 Mar 27:2023:2523536. PMID: 37020714 -
Food Chem Toxicol
PGC-1α/NRF1-dependent cardiac mitochondrial biogenesis: A druggable pathway of calycosin against triptolide cardiotoxicity. [Abstract]2023 Jan:171:113513. PMID: 36436616
SR-18292 purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2023 Jan:171:113513. [Abstract]
SR18292 (20 μM) successfully induces PGC-1α acetylation in both normal (fig A) and TP- and calycosin-treated cardiomyocytes (fig B).
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Physiol Behav
Exogenous IGF-1 alleviates depression-like behavior and hippocampal mitochondrial dysfunction in high-fat diet mice. [Abstract]2021 Feb 1:229:113236. PMID: 33137345 -
Connect Tissue Res
Dapagliflozin attenuates lipotoxic tenocyte injury via PPARα activation and irisin-driven antioxidant pathways. [Abstract]2025 Sep 17:1-13. PMID: 40959984 -
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Oxid Med Cell Longev
∆nFGF1 Protects β-Cells against High Glucose-Induced Apoptosis via the AMPK/SIRT1/PGC-1 α Axis. [Abstract]2022 Oct 3:2022:1231970. PMID: 36225175 -
Oxid Med Cell Longev
Cholecystokinin Octapeptide Promotes ANP Secretion through Activation of NOX4-PGC-1 α-PPAR α/PPAR γ Signaling in Isolated Beating Rat Atria. [Abstract]2022 Jun 20;2022:5905374. PMID: 35770043 -
Biomed Pharmacother
β2-adrenoreceptor agonist ameliorates mechanical allodynia in paclitaxel-induced neuropathic pain via induction of mitochondrial biogenesis. [Abstract]2021 Dec:144:112331. PMID: 34673421 -
Solvent & Solubility
DMSO : 100 mg/mL (272.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 100 mg/mL (272.85 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.82 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.82 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
For determination of GCN5 HAT activity U-2 OS cells overexpressing Ad-GCN5 are treated with SR-18292 (10 μM) for 18 h. Cells are lysed with buffer B (20 mM HEPES-KOH (pH 7.9), 125 mM NaCl, 1 mM EDTA, 1 mM DTT, 1% IGEPAL (v/v), 10% glycerol (v/v), 5 mM NaF, 5 mM β-glycerophosphate, 5 mM sodium butyrate and 10 mM nicotinamide), supplemented with Protease Inhibitor Cocktail. FLAG-GCN5 is immunoprecipitated with FLAG beads overnight at 4°C following multiple washes with lysis buffer. GCN5 is then eluted using 3× FLAG peptide and the purified protein is used to determine HAT activity using the HAT Inhibitor Screening Assay Kit[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
For cell viability determination using MTT, primary hepatocytes are seeded on a 96-well plate at 20,000 cells/well. The following day cells are treated at different doses, as indicated, for 18 h treatment of primary hepatocytes. 5 μL of MTT reagent (5 mg/mL) is then added to each well (n=4/dose) and cells are incubated for 1h at 37°C. Medium is discarded and dye is extracted by adding 100 μL DMSO to each well. For cytotoxicity determination using ToxiLight Non-destructive Cytotoxicity Bioassay, hepatocytes are seeded on a 6-well plate and treated with either SR-18292 (20 μM) or Cisplatin (50 μM) for 18 h. 50 μL of medium is collected and used to measure cellular toxicity by adding 100 of adenylate kinase detection reagent and incubating 5 min at RT before measuring luminescence[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[1]
For in vivo studies with DIO mice, males 6-8 weeks old are fed high fat diet (HFD) for the indicated time. For drug administration, SR-18292 (45 mg/kg) is injected via I.P. for 3 days between 4-5 pm and food is removed on day 3 at 5pm. The following morning (day 4) SR-18292 is injected again (for a total of 4 injections) and blood glucose is measured after 3 hours. Injection volume does not exceed 275 μL per mouse[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Ethanol | 1 mM | 2.7285 mL | 13.6426 mL | 27.2851 mL | 68.2128 mL |
| 5 mM | 0.5457 mL | 2.7285 mL | 5.4570 mL | 13.6426 mL | |
| 10 mM | 0.2729 mL | 1.3643 mL | 2.7285 mL | 6.8213 mL | |
| 15 mM | 0.1819 mL | 0.9095 mL | 1.8190 mL | 4.5475 mL | |
| 20 mM | 0.1364 mL | 0.6821 mL | 1.3643 mL | 3.4106 mL | |
| 25 mM | 0.1091 mL | 0.5457 mL | 1.0914 mL | 2.7285 mL | |
| 30 mM | 0.0910 mL | 0.4548 mL | 0.9095 mL | 2.2738 mL | |
| 40 mM | 0.0682 mL | 0.3411 mL | 0.6821 mL | 1.7053 mL | |
| 50 mM | 0.0546 mL | 0.2729 mL | 0.5457 mL | 1.3643 mL | |
| 60 mM | 0.0455 mL | 0.2274 mL | 0.4548 mL | 1.1369 mL | |
| 80 mM | 0.0341 mL | 0.1705 mL | 0.3411 mL | 0.8527 mL | |
| 100 mM | 0.0273 mL | 0.1364 mL | 0.2729 mL | 0.6821 mL |
SR-18292 Related Classifications
HY-101491 Related Classifications
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