1. Epigenetics
    TGF-beta/Smad
  2. PKC

(-)-Indolactam V (Synonyms: Indolactam V)

Cat. No.: HY-12307 Purity: 99.18%
Data Sheet SDS Handling Instructions

(-)-Indolactam V (ILV) is a protein kinase C activator, which strongly directs human ES cell-derived definitive endoderm into pancreatic endoderm.

For research use only. We do not sell to patients.
(-)-Indolactam V Chemical Structure

(-)-Indolactam V Chemical Structure

CAS No. : 90365-57-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO $365 In-stock
1 mg $150 In-stock
5 mg $550 In-stock
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50 mg   Get quote  

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Description

(-)-Indolactam V (ILV) is a protein kinase C activator, which strongly directs human ES cell-derived definitive endoderm into pancreatic endoderm. Target: PKC in vitro: Pancreatic endoderm induction by ILV alone has been shown to generate approximately 53% PDX1-positive cells, in contrast to 25% PDX1-positive cells achievable with standard inductors, FGF10, RA and CYC. Here, using human iPS-derived definitive endoderm, we demonstrated robust induction of pancreatic endoderm markers, PDX1, NGN3 and NEUROD1, only after inclusion of ILV to the FGF10, RA and CYC cocktail, underscoring the feasibility of generating pancreatic endoderm cells from human iPS cells through ILV-mediated guidance. It has been proposed that ILV may share an RA-dependent signaling pathway, allowing ultimately to streamline the process of efficient pancreatic endoderm derivation. [1] Due to the structural relatedness of the lyngbyatoxins and teleocidin, ILV was predicted to be a biosynthetic intermediate and was confirmed via in vitro prenylation by LtxC. The levels of ILV present in the heterologous host suggest an inefficient prenylation reaction with only 10% of ILV being converted to LTX.[2] (-)-Indolactam V induces differentiation of a substantial number of Pdx1-expressing cells from human ESCs. (-)-Indolactam V works specifically at one stage of pancreatic development, inducing pancreatic progenitors from definitive endoderm. (-)-Indolactam V (ILV), when combined with growth factors, can direct the differentiation of hESCs such that >45% of the cells become Pdx1-expressing pancreatic progenitors. [3]

References
M.Wt

301.38

Formula

C₁₇H₂₃N₃O₂

CAS No.

90365-57-4

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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(-)-Indolactam V
Cat. No.:
HY-12307
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