ABCB4 Antibody (YA7150)

Cat. No.: HY-P87463: Ficha de datos User Guide for Antibodies
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ABCB4 Antibody (YA7150) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to ABCB4.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

ABCB4 Antibody (YA7150) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to ABCB4.

Host

Mouse

Clonality

Monoclonal,Recombinant

Peso molecular

Predicted band size: 142kDa;

Species Reactivity

Human

SwissProt ID

P21439

Immunogen

Recombinant human ABCB4.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Sensitivity	Endogenous	Pureza	affinity purified by Protein G
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1, k

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed:17523162, PubMed:21820390, PubMed:23468132, PubMed:24594635, PubMed:24723470, PubMed:24806754, PubMed:31873305, PubMed:7957936, PubMed:8898203, PubMed:9366571). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes (PubMed:23468132). Required for proper phospholipid bile formation (By similarity). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed:24045840). Promotes biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed:28012258, PubMed:9366571). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed:21820390). Does not confer multidrug resistance (By similarity)

Subcellular Localization：Cell membrane,Apical cell membrane,Membrane raft,Cytoplasm,Cytoplasmic vesicle, clathrin-coated vesicle

Expression：Induction: Up-regulated by PPARA (PubMed:24122873). Up-regulated by compounds that cause peroxisome proliferation, such as fenofibrate (at protein level). Up-regulated by bezafibrate (PubMed:15258199). Up-regulated by compounds that cause peroxisome proliferation, such as fenofibrate, bezafibrate and gemfibrozil (PubMed:24122873)

Isoforms & Post-Translational Modification：P21439 has three isomers: P21439-1: 141523 Da (predicted); P21439-2: 140682 Da (predicted); P21439-3: 135259 Da (predicted).
Phosphorylated (PubMed:24723470)丨Glycosylated (PubMed:17523162, PubMed:21820390, PubMed:24723470)

Subunit：May interact with RACK1 (PubMed:19674157)

Documentación

User Guide for Antibodies (1077 KB)