Aquaporin 1 Antibody (YA1149)(PBS only)

Cat. No.: HY-P81404A: Data Sheet User Guide for Antibodies
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Aquaporin 1 Antibody (YA1149) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Aquaporin 1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Aquaporin 1 Antibody (YA1149) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Aquaporin 1.

Host

Rabbit

Molecular Weight

Predicted band size: 29 kDa;

Observed band size: 29 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P29972

Gene ID

358 [NCBI]

Immunogen

A synthesized peptide derived from human Aquaporin 1

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Forms a water channel that facilitates the transport of water across cell membranes, playing a crucial role in water homeostasis in various tissues (PubMed:1373524, PubMed:23219802). Could also be permeable to small solutes including hydrogen peroxide, glycerol and gases such as amonnia (NH3), nitric oxide (NO) and carbon dioxide (CO2) (PubMed:16682607, PubMed:17012249, PubMed:19273840, PubMed:33028705, PubMed:8584435). Recruited to the ankyrin-1 complex, a multiprotein complex of the erytH2O2yte membrane, it could be part of a CO2 metabolon, linking facilitated diffusion of CO2 across the membrane, anion exchange of Cl(-)/H2O2(-) and interconversion of dissolved CO2 and carbonic acid in the cytosol (PubMed:17012249, PubMed:35835865). In vitro, it shows non-selective gated cation channel activity and may be permeable to cations like K(+) and Na(+) in vivo (PubMed:36949749, PubMed:8703053)

Subcellular Localization：Cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Detected in erytH2O2ytes (at protein level) . Expressed in a number of tissues including erytH2O2ytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver

Isoforms & Post-Translational Modification：P29972 has 4 isomers: P29972-1: 28526 Da (predicted); P29972-2: 19956 Da (predicted); P29972-3: 23425 Da (predicted); P29972-4: 16677 Da (predicted).

Subunit：Homotetramer; each monomer provides an independent water pore (PubMed:11171962, PubMed:35835865). Component of the ankyrin-1 complex in the erytH2O2yte, composed of ANK1, RHCE, RHAG, SLC4A1, EPB42, GYPA, GYPB and AQP1 (PubMed:35835865). Interacts with EPHB2; involved in endolymph production in the inner ear (By similarity). Identified in a complex with STOM (PubMed:23219802). Interacts (via the N-terminal) with ANK1 (via ANK 1-5 repeats) (PubMed:35835865). Interacts (via the C-terminal) with EPB42 (PubMed:35835865)

Research Field

Cell Biology

Synonyms

AQP1; CHIP28; Aquaporin-1; AQP-1; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule

Documentation

User Guide for Antibodies (1077 KB)