ATG14/ATG14L Antibody (YA7058)

Cat. No.: HY-P87375: Data Sheet User Guide for Antibodies
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ATG14/ATG14L Antibody (YA7058) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATG14/ATG14L.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈	가격	재고
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

ATG14/ATG14L Antibody (YA7058) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATG14/ATG14L.

Host

Mouse

Clonality

Monoclonal

분자량

Predicted band size: 55.3kDa;

Observed band size: 55.3kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q6ZNE5

Immunogen

Purified recombinant fragment of human ATG14L (AA: 43-303) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604, PubMed:37632749). Modulates the hepatic lipid metabolism (By similarity)

Subcellular Localization：Cytoplasm,Endoplasmic reticulum membrane,Preautophagosomal structure membrane,Cytoplasmic vesicle, autophagosome membrane

Isoforms & Post-Translational Modification：Q6ZNE5 has two isomers: Q6ZNE5-1: 55309 Da (predicted); Q6ZNE5-2: 42434 Da (predicted).
Ubiquitinated via 'Lys-6', 'Lys-11' and 'Lys-63'-linked polyubiquitin chains on multiple lysines by MARCHF7, leading to ATG14 aggregation and loss of interaction with STX17

Subunit：Forms homooligomers; homo-oligomerization is essential for the roles in membrane tethering and enhancement of SNARE-mediated fusion (PubMed:25686604)

Synonyms

1110038I05Rik antibody; AI314632 antibody; Aldehyde dehydrogenase 6 family member A1 antibody; Aldehyde dehydrogenase family 6 member A1 antibody; Aldehyde dehydrogenase family 6, subfamily A1 antibody; ALDH6A1 antibody; Malonate semialdehyde dehydrogenase antibody; Malonate-semialdehyde dehydrogenase [acylating] antibody; Methylmalonate semialdehyde dehydrogenase [acylating] mitochondrial antibody; Methylmalonate-semialdehyde dehydrogenase [acylating] antibody

각종 서류

User Guide for Antibodies (1077 KB)