Bim Antibody (YA6018)

Cat. No.: HY-P86326: Ficha de datos User Guide for Antibodies
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Bim Antibody (YA6018) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bim.

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Tamaño	Precio	Stock
20 μL		Pide un presupuesto y un plazo de entrega
50 μL		Pide un presupuesto y un plazo de entrega
100 μL		Pide un presupuesto y un plazo de entrega

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

Bim Antibody (YA6018) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bim.

Host

Rabbit

Clonality

Monoclonal

Peso molecular

Predicted band size: 22 kDa;

Observed band size: 22 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O43521

Gene ID

10018 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Pureza	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of MCF7 cells labeling Bim Antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bim Antibody at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of MCF7 cells labeling Bim Antibodyat 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bim Antibody at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis

Subcellular Localization：Endomembrane system; Peripheral membrane protein; Mitochondrion; Mitochondrion; Mitochondrion; Mitochondrion

Expression：
Tissue_specificity:The three main subtypes, BimEL, BimL, and BimS, are widely expressed, but exhibit tissue-specific differences. The Bim-γ subtype is most abundantly expressed in the small intestine and colon, with lower expression levels in the spleen, prostate, testis, heart, liver, and kidney.

Induction:By ER stress in a DDIT3/CHOP-dependent manner

Isoforms & Post-Translational Modification：O43521 has 20 isomers: O43521-1: 22171 Da (predicted); O43521-2: 15967 Da (predicted); O43521-3: 12717 Da (predicted); O43521-4: 18536 Da (predicted); O43521-5: 12332 Da (predicted); O43521-6: 9081 Da (predicted); O43521-7: 9596 Da (predicted); O43521-8: 18816 Da (predicted); O43521-9: 9362 Da (predicted); O43521-10: 14458 Da (predicted); O43521-11: 14418 Da (predicted); O43521-12: 22649 Da (predicted); O43521-13: 4834 Da (predicted); O43521-14: 15025 Da (predicted); O43521-15: 8214 Da (predicted); O43521-16: 8821 Da (predicted); O43521-17: 12412 Da (predicted); O43521-18: 11773 Da (predicted); O43521-19: 17977 Da (predicted); O43521-20: 8523 Da (predicted).
Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation (PubMed:15486195, PubMed:21478148). Deubiquitination catalyzed by USP27X stabilizes the protein (By similarity);Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein

Subunit：Forms heterodimers with a number of antiapoptotic Bcl-2 proteins, including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, BHRF1, and BCL2L2/BCLW (PubMed:11997495, PubMed:18812174, PubMed:27013495). Does not heterodimerize with proapoptotic proteins such as BAD, BOK or BAK. Identified in a complex containing BCL2L11, DYNLL1 and BCL2L1 isoform Bcl-X(L); BH3 integrity is required for BCL2L1-binding. Interacts with YWHAZ. When phosphorylated, interacts with TRIM2; this interaction is associated with ubiquitination and degradation (PubMed:21478148). Interacts with MCL1; may sequester BCL2L11 to prevent its pro-apoptotic activity (PubMed:17389404, PubMed:27013495). Interacts with GIMAP5 (PubMed:16509771). Interacts with BCL2L10/BCL-B (PubMed:22498477, PubMed:23235460, PubMed:23563182)

Synonyms

BCL2L11; BIM; Bcl-2-like protein 11; Bcl2-L-11; Bcl2-interacting mediator of cell death

Documentación

User Guide for Antibodies (1077 KB)