CEP63 Antibody (YA8767)(PBS only)

Cat. No.: HY-P89083A: Fiche technique User Guide for Antibodies
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CEP63 Antibody (YA8767) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CEP63.

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Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description
Références

Description

CEP63 Antibody (YA8767) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CEP63.

Host

Mouse

Masse moléculaire

Predicted band size: 62.8 kDa

Species Reactivity

Human

SwissProt ID

Q96MT8

Immunogen

Full length human recombinant protein of human CEP63 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-800

Pureté	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Required for normal spindle assembly (PubMed:21406398, PubMed:21983783, PubMed:26297806, PubMed:35793002). Plays a key role in mother-centriole-dependent centriole duplication; the function seems also to involve CEP152, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication (PubMed:21983783, PubMed:26297806). Reported to be required for centrosomal recruitment of CEP152; however, this function has been questioned (PubMed:21983783, PubMed:26297806). Also recruits CDK1 to centrosomes (PubMed:21406398). Plays a role in DNA damage response (PubMed:21406398). Following DNA damage, such as double-strand breaks (DSBs), is removed from centrosomes; this leads to the inactivation of spindle assembly and delay in mitotic progression (PubMed:21406398). Promotes stabilization of FXR1 protein by inhibiting FXR1 ubiquitination (PubMed:35989368)

Subcellular Localization：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite

Isoforms & Post-Translational Modification：Q96MT8 has four isomers: Q96MT8-1: 81344 Da (predicted); Q96MT8-2: 63015 Da (predicted); Q96MT8-3: 57908 Da (predicted); Q96MT8-4: 55591 Da (predicted).
Polyubiquitinated via 'Lys-48'-linked ubiquitin, leading to its degradation (PubMed:35989368)

Subunit：Interacts with CEP152 and CDK1; these interactions recruit both ligands to centrosomes (PubMed:21406398)

Synonyms

SCKL6

Documentation

User Guide for Antibodies (1077 KB)

Références

[1]. /biology-dictionary/vero-c1008-cell.html