CYP2B6 (Cytochrome P450 2B6) Antibody (YA7956)

Cat. No.: HY-P88272: Data Sheet User Guide for Antibodies
FAQs
Technical Support

CYP2B6 (Cytochrome P450 2B6) Antibody (YA7956) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CYP2B6 (Cytochrome P450 2B6).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CYP2B6 (Cytochrome P450 2B6) Antibody (YA7956) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CYP2B6 (Cytochrome P450 2B6).

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 56.1 kDa

Species Reactivity

Human, Dog, Rat, Monkey, Mouse

SwissProt ID

P20813

Immunogen

Full length human recombinant protein of human CYP2B6 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids (PubMed:12865317, PubMed:21289075). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed:21289075). Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2 (PubMed:12865317, PubMed:21289075). Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs (PubMed:11695850, PubMed:22909231). Acts as a 1,4-cineole 2-exo-monooxygenase (PubMed:11695850)|Allele 2B6*9: Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs

Subcellular Localization：Endoplasmic reticulum membrane,Microsome membrane

Expression：
Tissue_Specificity: Expressed in liver, lung and heart right ventricle
Induction: By phenobarbital

Isoforms & Post-Translational Modification：P20813 has two isomers: P20813-1: 56278 Da (predicted); P20813-2: 33385 Da (predicted).
Phosphorylation is accompanied by a decrease in enzyme activity

Synonyms

CPB6; CYP2B; CYP2B7; CYP2B7P; CYPIIB6; EFVM; IIB1; P450

Documentation

User Guide for Antibodies (1077 KB)