DICER1 Antibody (YA7742)

Cat. No.: HY-P88058: Data Sheet User Guide for Antibodies
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DICER1 Antibody (YA7742) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DICER1.

For research use only. We do not sell to patients.

Size	Price	Stock
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DICER1 Antibody (YA7742) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DICER1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 218.5 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9UPY3

Immunogen

Human recombinant protein fragment corresponding to amino acids 1666-1922 of human DICER1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes

Subcellular Localization：Cytoplasm,Cytoplasm, perinuclear region

Isoforms & Post-Translational Modification：Q9UPY3 has three isomers: Q9UPY3-1: 218682 Da (predicted); Q9UPY3-2: 208448 Da (predicted); Q9UPY3-3: 92726 Da (predicted).

Subunit：Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2

Synonyms

DCR1; Dicer; Dicer1e; HERNA; MNG1; RMSE2

Documentation

User Guide for Antibodies (1077 KB)