IGFBP3 Antibody (YA7144)

Cat. No.: HY-P87457: Data Sheet User Guide for Antibodies
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IGFBP3 Antibody (YA7144) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

IGFBP3 Antibody (YA7144) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP3.

Host

Rabbit

Clonality

Recombinant

Molecular Weight

Predicted band size: 30-35kDa;

Species Reactivity

Human

SwissProt ID

P17936

Immunogen

KLH conjugated synthetic peptide derived from human IGFBP3

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:10874028, PubMed:19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed:20353938). Inhibits the positive effect of humanin on insulin sensitivity (PubMed:19623253). Promotes testicular germ cell apoptosis (PubMed:19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed:9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed:15371331). In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed:10874028)

Subcellular Localization：Secreted,Nucleus

Expression：
Tissue_Specificity: Expressed by most tissues. Present in plasma
Induction: Up-regulated in the presence of IGF1, insulin and other growth-stimulating factors such as growth hormone, EGF and phorbol esters

Isoforms & Post-Translational Modification：P17936 has two isomers: P17936-1: 31674 Da (predicted); P17936-2: 32223 Da (predicted).
Phosphorylated by FAM20C in the extracellular medium

Subunit：Interacts with XLKD1 (By similarity)

Documentation

User Guide for Antibodies (1077 KB)