Human IgG3 Antibody (YA9054)

Cat. No.: HY-P89710: Data Sheet User Guide for Antibodies
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Human IgG3 Antibody (YA9054) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Human IgG3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Human IgG3 Antibody (YA9054) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Human IgG3.

Host

Mouse

Molecular Weight

Predicted band size: 41 kDa

Species Reactivity

human

SwissProt ID

P01860

Gene ID

3502 [NCBI]

Immunogen

A synthesized peptide derived from human IgG3.

Application &
Dilution Ratio

Application	Dilution Ratio
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:50-3000

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Solution

Formulation

Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

Storage & Stability

Stored at 2-8°C for 1 year, do not freeze.

Shipping

Shipping with blue ice.

Background

Function：Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414).

Subcellular Localization：Secreted; Cell membrane; Single-pass membrane protein

Isoforms & Post-Translational Modification：P01860 has 2 isomers: P01860-1: 41,287 Da (predicted); P01860-2: 49,093 Da (predicted).
N-glycosylated. The core glycan is composed of two sequential N-acetylglucosamine (GlcNAc) moieties followed by a central mannose (Man) from which two additional Man residues branch out (alpha1,3 and alpha1,6 antennae) each capped with a GlcNAc. Variable addition of sugars account for different IgG functional states. N-linked glycans at Asn-322 are noncore fucosylated and the vast majority are diantennary species with a bisecting GlcNAc. (Microbial infection) Deglycosylation on Asn-227 by S.pyogenes EndoS or Endos2 endoglucosidases prevents interaction between immunoglobulin-gamma (IgG) and Fc receptors, impairing ability to activate the complement pathway. O-linked glycans are non-, mono- and disialylated core 1-type O-glycans.

Subunit：Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked

Database

OMIM: 147120

Synonyms

IGHG3, IgG3，Human igg3

Documentation

User Guide for Antibodies (1077 KB)