Nkx-2.5 Antibody (YA4989)

Cat. No.: HY-P85297: Data Sheet User Guide for Antibodies
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Nkx-2.5 Antibody (YA4989) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Nkx-2.5.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Nkx-2.5 Antibody (YA4989) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Nkx-2.5.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 35 kDa;

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P52952

Gene ID

1482 [NCBI]

Immunogen

Purified recombinant fragment of human Nkx-2.5 expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:200-1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-500
IP IP: Immunoprecipitation	1-2μg per 100-500μg Total protein
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:50-3000

Sensitivity	Endogenous	Purity	affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

Storage & Stability

Stored at 2-8°C for 1 year, do not freeze.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor required for the development of the heart and the spleen (PubMed:22560297). During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4 (By similarity). May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal (By similarity). Binds to the core DNA motif of NPPA promoter (PubMed:22849347, PubMed:26926761). Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression (PubMed:22560297). Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia (PubMed:29899023)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Expressed only in the heart

Isoforms & Post-Translational Modification：P52952 has 3 isomers: P52952-1: 34918 Da (predicted); P52952-2: 16102 Da (predicted); P52952-3: 11681 Da (predicted).

Subunit：Homodimer (via the homeobox); binds DNA as homodimer (PubMed:22849347). Interacts (via the homeobox) with TBX5 (via the T-box); this complex binds DNA (PubMed:26926761). Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Interacts with TBX18 (By similarity). Interacts with histone methyltransferase NSD2 (via HMG box) (By similarity). Interacts with NEDD9 (PubMed:29899023). Interacts with TBX1 (By similarity)

Synonyms

NKX2-5; CSX; NKX2.5; NKX2E; Homeobox protein Nkx-2.5; Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mcf7-cell.html