PA28γ Antibody (YA5139)

Cat. No.: HY-P85447: Data Sheet User Guide for Antibodies
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PA28γ Antibody (YA5139) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PA28γ.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PA28γ Antibody (YA5139) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PA28γ.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 30 kDa;

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P61290

Gene ID

10197 [NCBI]

Immunogen

Purified recombinant mouse PA28γ protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:200-1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-500
IP IP: Immunoprecipitation	1-2μg per 100-500μg Total protein
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:50-3000

Sensitivity	Endogenous	Purity	affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

Storage & Stability

Stored at 2-8°C for 1 year, do not freeze.

Shipping

Shipping with blue ice.

Background

Function：Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Induction:Up-regulated in thyroid carcinoma cells

Isoforms & Post-Translational Modification：P61289 has 3 isomers: P61289-1: 29506 Da (predicted); P61289-2: 30887 Da (predicted); P61289-3: 30890 Da (predicted).
Phosphorylated by MAP3K3 (By similarity). Phosphorylation at Ser-247 promotes its association with CCAR2;Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1

Subunit：Homoheptamer; the stability of the heptamer is essential for the specific activation of the trypsine-like subunit and inhibition of the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits of the proteasome. Interacts with p53/TP53 and MDM2. Interacts with MAP3K3 (By similarity). Associates with the proteasome. Interacts with CCAR2. Interacts with PSME3IP1 (via C-terminus); the interaction is direct and promotes the association of PSME3 with the 20S proteasome (PubMed:29934401). Interacts with COIL; the interaction is inhibited by PSME3IP1 (PubMed:29934401)

Synonyms

PSME3; Proteasome activator complex subunit 3; 11S regulator complex subunit gamma; REG-gamma; Activator of multicatalytic protease subunit 3; Ki nuclear autoantigen; Proteasome activator 28 subunit gamma; PA28g; PA28gamma

Documentation

User Guide for Antibodies (1077 KB)