PARL Antibody (YA5007)

Cat. No.: HY-P85315: Data Sheet User Guide for Antibodies
FAQs
Technical Support

PARL Antibody (YA5007) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PARL.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PARL Antibody (YA5007) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PARL.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 42 kDa;

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9H300

Gene ID

55486 [NCBI]

Immunogen

Purified recombinant fragment of PARL (aa80-371) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:200-1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-500
IP IP: Immunoprecipitation	1-2μg per 100-500μg Total protein
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:50-3000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS with < 0.1% sodium azide and 0.1% gelatin.

Storage & Stability

Stored at 2-8°C for 1 year, do not freeze.

Shipping

Shipping with blue ice.

Background

Function：Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals (By similarity). Required for the maturation of PINK1 into its 52kDa mature form after its cleavage by mitochondrial-processing peptidase (MPP) (PubMed:22354088). Promotes cleavage of serine/threonine-protein phosphatase PGAM5 in damaged mitochondria in response to loss of mitochondrial membrane potential (PubMed:22915595). Mediates differential cleavage of PINK1 and PGAM5 depending on the health status of mitochondria, disassociating from PINK1 and associating with PGAM5 in response to mitochondrial membrane potential loss (PubMed:22915595). Required for processing of CLPB into a form with higher protein disaggregase activity by removing an autoinhibitory N-terminal peptide (PubMed:28288130, PubMed:32573439). Promotes processing of DIABLO/SMAC in the mitochondrion which is required for DIABLO apoptotic activity (PubMed:28288130). Also required for cleavage of STARD7 and TTC19 (PubMed:28288130). Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain (PubMed:14732705, PubMed:17116872)

Subcellular Localization：Mitochondrion inner membrane; Multi-pass membrane protein; Nucleus

Isoforms & Post-Translational Modification：Q9H300 has 2 isomers: Q9H300-1: 42190 Da (predicted); Q9H300-2: 36589 Da (predicted).
P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner. The cleavage is inhibited when residues Ser-65, Thr-69 and Ser-70 are all phosphorylated

Subunit：Interacts with PSEN1 and PSEN2. Binds OPA1

Synonyms

PARL; PSARL; Presenilins-associated rhomboid-like protein; mitochondrial; Mitochondrial intramembrane cleaving protease PARL

Documentation

User Guide for Antibodies (1077 KB)