PAX5 Antibody (YA5712)

Cat. No.: HY-P86020: Data Sheet User Guide for Antibodies
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PAX5 Antibody (YA5712) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to PAX5.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

PAX5 Antibody (YA5712) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to PAX5.

Host

Mouse

Clonality

Monoclonal

분자량

Predicted band size: 42 kDa;

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q02548

Gene ID

5079 [NCBI]

Immunogen

Synthesized peptide derived from human PAX-5 AA range:300-383

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:500-5000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed:10811620, PubMed:27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed:10811620, PubMed:27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed:32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed:32612238); (Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription (PubMed:31941781). Also participates in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity (PubMed:23678172)

Subcellular Localization：Nucleus

Isoforms & Post-Translational Modification：Q02548 has 11 isomers: Q02548-1: 42149 Da (predicted); Q02548-2: 38890 Da (predicted); Q02548-3: 35281 Da (predicted); Q02548-4: 32471 Da (predicted); Q02548-5: 31830 Da (predicted); Q02548-6: 38773 Da (predicted); Q02548-7: 35514 Da (predicted); Q02548-8: 37834 Da (predicted); Q02548-9: 34574 Da (predicted); Q02548-10: 31381 Da (predicted); Q02548-11: 34132 Da (predicted).
O-glycosylated;Phosphorylated by SYK. This phosphorylation plays an important role in the abolition of BLIMP1 repression by PAX5 in order to trigger plasma cell differentiation

Subunit：Interacts with ETS1; this interaction alters PAX5 DNA-binding properties (PubMed:11779502). Binds DNA as a monomer (PubMed:11779502). Interacts with TBP; this interaction allows PAX5 to interact with the basal transcription machinery (PubMed:10197586). Interacts with RB1 (PubMed:10197586). Interacts with TLE4 (PubMed:10811620). Interacts with DAXX (By similarity)

Synonyms

Paired box protein Pax-5; B-cell-specific transcription factor; BSAP;

각종 서류

User Guide for Antibodies (1077 KB)