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PKN1 Antibody (YA4308)

(Synonyms: DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA)
Customer Review

Based on 1 Customer Validation

PKN1 Antibody (YA4308) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PKN1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL IHC-P FC ICC
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • PKN1 Antibody (YA4308) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

PKN1 Antibody (YA4308) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PKN1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 120 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 104 kDa
Immunogen

Purified recombinant fragment of human PKN1 (AA: 442-620) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718949

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PKN1 Antibody (YA4308)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    Immunohistochemical analysis of paraffin-embedded human stomach cancer using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    Immunohistochemical analysis of paraffin-embedded human prostate cancer using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    Flow cytometric analysis of 1X106 HeLa cells labeling PKN1 Antibody (HY-P84611, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    Flow cytometric analysis of 1X10^6 Hela cells labeling PKN1 Antibody(HY-P84611
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    Immunofluorescence analysis of Hela cells labeling PKN1 antibody (HY-P84611) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

Background

  • Function

    PKN1 (protein kinase N1, PRK1) is a member of the protein kinase N family of AGC serine/threonine kinases that functions downstream of Rho family GTPases and phospholipid-dependent signaling to regulate cytoskeletal organization, cell migration, transcriptional control, and cell survival pathways[1][4]. Mechanistically, PKN1 links RhoA-dependent signaling to phosphorylation events that influence intermediate filament dynamics, actin-associated structures, and downstream signaling networks including PI3K/AKT-related processes[1][2]. Through these activities, PKN1 contributes to the regulation of cellular proliferation and differentiation, and genetic or functional perturbation of PKN1 alters signaling responses in multiple experimental systems[2][3]. In disease-relevant contexts, PKN1 has been associated with tumor cell invasion, androgen receptor-dependent transcriptional regulation, and cancer-related signaling pathways, supporting its use as a target for mechanistic studies of oncogenic signaling and cytoskeletal remodeling[4][5]. Compared with related isoforms, PKN1 and PKN2 are broadly expressed across tissues, whereas PKN3 displays a more restricted expression pattern and has been linked more specifically to tumor-associated functions, highlighting important isoform-specific biology within the PKN family[3]. For experimental applications, pharmacological kinase inhibitors and genetic loss-of-function approaches have been used to define PKN1-dependent signaling networks, enabling investigation of isoform-selective functions and evaluation of PKN1 as a potential therapeutic target in cancer and other disease models[2][3].

  • Subcellular Localization

    Cytoplasm; Nucleus; Endosome; Cell membrane; Peripheral membrane protein; Cleavage furrow; Midbody

  • Expression


    Tissue_specificity:It is widely present in various tissues. It is expressed in the heart, brain, placenta, lungs, skeletal muscle, kidneys, and pancreas. It is also expressed in a variety of tumor cell lines, especially breast tumor cells.

  • Subunit

    Interacts with ZFAND6 (By similarity). Interacts with AR (PubMed:12514133). Interacts with PRKCB (PubMed:20228790). Interacts (via REM 1 and REM 2 repeats) with RAC1 (PubMed:14514689, PubMed:18006505). Interacts (via REM 1 repeat) with RHOA (PubMed:10619026, PubMed:8571126). Interacts with RHOB (PubMed:9478917). Interacts (via C-terminus) with PDPK1 (PubMed:10792047). Interacts with CCNT2; enhances MYOD1-dependent transcription (PubMed:16331689). Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (PubMed:21224381)

  • SwissProt ID

    Q16512

  • Gene ID
    5585 [NCBI]
  • Synonyms

    DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA

References

[1]. Uniprotkb.

[2]. Yasui T, et al. Protein kinase N1, a cell inhibitor of Akt kinase, has a central role in quality control of germinal center formation. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):21022-7. [Content Brief]

[3]. Falk MD, et al. Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors. Biosci Rep. 2014;34(2):e00097. [Content Brief]

[4]. Sophocleous G, et al. The structure and function of protein kinase C-related kinases (PRKs). Biochem Soc Trans. 2021;49(1):217-235.

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PKN1 Antibody (YA4308) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach cancer using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling PKN1 Antibody (HY-P84611, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling PKN1 Antibody(HY-P84611

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling PKN1 antibody (HY-P84611) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • PKN1 Antibody (YA4308) Product Image
    1/16
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • PKN1 Antibody (YA4308) Product Image
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach cancer using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling PKN1 Antibody (HY-P84611, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling PKN1 Antibody(HY-P84611
  • Experimental Validation Results for PKN1 Antibody (YA4308)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling PKN1 antibody (HY-P84611) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • Experimental Validation Results for PKN1 Antibody (YA4308)
  • PKN1 Antibody (YA4308) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PKN1 Antibody (YA4308) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PKN1 Antibody (YA4308) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PKN1 Antibody (YA4308) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PKN1 Antibody (YA4308) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PKN1 Antibody (YA4308) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using PKN1 Antibody (HY-P84611, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PKN1 Antibody (YA4308) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling PKN1 Antibody (HY-P84611, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • PKN1 Antibody (YA4308) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling PKN1 Antibody(HY-P84611

  • PKN1 Antibody (YA4308) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling PKN1 antibody (HY-P84611) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • PKN1 Antibody (YA4308) Product Image IHC-P
  • PKN1 Antibody (YA4308) Product Image IHC-P
  • PKN1 Antibody (YA4308) Product Image IHC-P
  • PKN1 Antibody (YA4308) Product Image IHC-P
  • PKN1 Antibody (YA4308) Product Image IHC-P
  • PKN1 Antibody (YA4308) Product Image IHC-P
  • PKN1 Antibody (YA4308) Product Image FC
  • PKN1 Antibody (YA4308) Product Image FC
  • PKN1 Antibody (YA4308) Product Image ICC