Slingshot homolog 1 Antibody (YA6972)

Cat. No.: HY-P87289: Ficha de datos COA
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Slingshot homolog 1 Antibody (YA6972) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Slingshot homolog 1.

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Tamaño	Stock	Cantidad
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

Slingshot homolog 1 Antibody (YA6972) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Slingshot homolog 1.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 115.5kDa;

Observed band size: 115.5kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q8WYL5

Immunogen

Synthesized peptide fragment of human SLINGSHOT-1L (AA: 1032-1044 ) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Pureza	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Solution

Formulation

Supplied in in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Flow cytometric analysis of 1X10^6 Hela cells labeling SLINGSHOT-1L Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein

Subcellular Localization：Cytoplasm, cytoskeleton,Cell projection, lamellipodium,Cleavage furrow,Midbody

Isoforms & Post-Translational Modification：Q8WYL5 has 5 isomers: Q8WYL5-1: 115511 Da (predicted); Q8WYL5-2: 77430 Da (predicted); Q8WYL5-3: 16695 Da (predicted); Q8WYL5-4: 80684 Da (predicted); Q8WYL5-5: 78978 Da (predicted).
Phosphorylated

Subunit：Interacts with actin and this stimulates phosphatase activity

Synonyms

AW551225 antibody; Coro1c antibody; coronin; actin binding protein 1C antibody; Gm1394 antibody; Gm1395 antibody; hSSH-1L antibody; mSSH 1L antibody; OTTMUSP00000031415 antibody; Protein phosphatase Slingshot homolog 1 antibody

Documentación

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Ficha de datos (230 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)