SUPT16H Antibody (YA7888)

Cat. No.: HY-P88204: Ficha de datos COA
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SUPT16H Antibody (YA7888) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SUPT16H.

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Tamaño	Stock	Cantidad
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn
Referencias

Descripciòn

SUPT16H Antibody (YA7888) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SUPT16H.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9Y5B9

Immunogen

Human recombinant protein fragment corresponding to amino acids 187-282 of human SUPT16H produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Pureza	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A549 (lane2(20μg), MCF-7 (lane3(20μg), Hela (lane4(20μg) and Mouse brain tissue (lane5(20μg) using SUPT16H Antibody (HY-P88204). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

Background

Function：Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II)

Subcellular Localization：Nucleus,Chromosome

Expression：
Tissue_Specificity: Ubiquitous

Isoforms & Post-Translational Modification：Q9Y5B9: 1047 amino acids, molecular weight 119914 Da.

Subunit：Interacts with MYOG (via C-terminal region) (By similarity)

Synonyms

CDC68; FACTP140; SPT16; SPT16/CDC68

Documentación

Select Batch:

Ficha de datos (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/mda-mb-231-cell.html